School of Pharmacy and Medical Sciences, University of South Australia, GPO Box 2471, Adelaide, South Australia 5001, Australia.
J Pharm Biomed Anal. 2011 Jan 25;54(2):303-10. doi: 10.1016/j.jpba.2010.08.024. Epub 2010 Sep 24.
Allergy to Myrmecia pilosula (Jack Jumper Ant) venom is common in Australia, affecting ∼2.7% of some communities. Venom immunotherapy is a highly effective treatment, but for the venom to be widely distributed for clinical use, the stability and shelf-life of formulated Jack Jumper Ant venom must be demonstrated. HPLC-UV, ELISA Inhibition, SDS-PAGE and SDS-PAGE Immunoblot were used to assess venom stability under conditions of varying temperature, pH and in the presence of various stabilising agents. Optimal stability occurred between pH 8 and 10, however the presence of benzyl alcohol within this pH range resulted in a cloudy appearance within 3 days, so a pH of 6 was used. Increasing polysorbate 80 concentrations accelerated the degradation of allergenic peptides in 100 μg/mL venom, but improved stability at concentrations of 1 μg/mL or less. Sucrose reduced degradation of allergens Myr p 1 and Myr p 3, whilst glycerol was destabilizing. In the presence of 22% sucrose, 1.1mg/mL Jack Jumper Ant venom was stable at -18 °C and 4 °C for 12 months; following dilution to 100 μg/mL with 0.9% sodium chloride, 10mM phosphate (pH 6), 0.05% polysorbate 80 and 0.9% benzyl alcohol (giving 2% sucrose), venom was stable for 7 days when stored at 4 °C. Concentrated Jack Jumper Ant venom can be stored in 22% sucrose for 12 months, and after dilution to 100 μg/mL for clinical use, it should be discarded after 7 days.
对 Myrmecia pilosula（杰克跳蚁）毒液的过敏在澳大利亚很常见，影响了一些社区中约 2.7%的人群。毒液免疫疗法是一种非常有效的治疗方法，但为了使毒液广泛用于临床，必须证明已配制的杰克跳蚁毒液的稳定性和保质期。使用 HPLC-UV、ELISA 抑制、SDS-PAGE 和 SDS-PAGE 免疫印迹来评估在不同温度、pH 值和存在各种稳定剂的条件下毒液的稳定性。最佳稳定性发生在 pH 值 8 到 10 之间，但在该 pH 值范围内存在苯甲醇会导致在 3 天内出现混浊，因此使用 pH 值 6。增加聚山梨醇酯 80 的浓度会加速 100μg/mL 毒液中变应原肽的降解，但在 1μg/mL 或更低的浓度下会提高稳定性。蔗糖减少了过敏原 Myr p 1 和 Myr p 3 的降解，而甘油则使稳定性降低。在 22%蔗糖存在的情况下，1.1mg/mL 的杰克跳蚁毒液在-18°C 和 4°C 下可稳定 12 个月；用 0.9%氯化钠、10mM 磷酸盐（pH 6）、0.05%聚山梨醇酯 80 和 0.9%苯甲醇（2%蔗糖）稀释至 100μg/mL 后，在 4°C 下储存时，毒液可稳定 7 天。浓缩的杰克跳蚁毒液可在 22%蔗糖中储存 12 个月，稀释至 100μg/mL 用于临床后，应在 7 天后丢弃。
