University of Twente, Institute for Biomedical Technology, Department of Electrical Engineering, The Netherlands.
J Electromyogr Kinesiol. 1994;4(1):37-46. doi: 10.1016/1050-6411(94)90025-6.
Single muscle fibre action potentials (SFAPs) are considered to be functions of a bioelectrical source and electrical conductivity parameters of the medium. In most model studies SFAPs are computed as a convolution of the bioelectrical source with a transfer function. Calculated peak-to-peak amplitudes of SFAPs decrease with increasing recording distances. In this paper an experimental validation of model results is presented. Experiments were carried out on the m. extensor digitorum longus (EDL) of the rat. Using a method including fluorescent labelling of the active fibre, the distance between the active fibre and the recording electrode was derived. With another method, the decline of the peak-to-peak amplitude of SFAPs detected along a multi-electrode was obtained. With both experimental methods, in general peak-to-peak amplitudes of SFAPs decreased with increasing recording distances, as was found in model results with present volume conduction theory. However, this behaviour was not found in all experiments. The rate of decline of the peak-to-peak amplitudes with recording distance was always less than in models.
单肌纤维动作电位（SFAPs）被认为是生物电源和介质电导率参数的函数。在大多数模型研究中，SFAPs 被计算为生物电源与传递函数的卷积。随着记录距离的增加，SFAPs 的峰峰值幅度减小。本文介绍了对模型结果的实验验证。实验在大鼠的 m. extensor digitorum longus (EDL)上进行。使用一种包括活性纤维荧光标记的方法，得出了活性纤维和记录电极之间的距离。通过另一种方法，获得了沿着多电极检测到的 SFAPs 峰峰值幅度的衰减。使用这两种实验方法，一般来说，随着记录距离的增加，SFAPs 的峰峰值幅度减小，这与目前的容积传导理论的模型结果一致。然而，并非所有实验都存在这种情况。与模型相比，峰峰值幅度随记录距离的衰减速率总是较小。
