Jurkiewicz A, Caricati-Neto A, Brito A R, Mulford S S, Busatto P A
Department of Pharmacology, Escola Paulista de Medicina, Sao Paulo, Brazil.
Methods Find Exp Clin Pharmacol. 1990 Sep;12(7):473-9.
A device for double perfusion of the vas deferens externally and through the lumen is described in detail. The perfusion system allows the simultaneous recording of drug-induced or spontaneous contractions of the circular and longitudinal smooth muscle layers of the organ. Isometric contractions of the longitudinal (external) layer are recorded through a tension transducer. The contractions of the circular (internal) smooth muscle layer are recorded as changes of the pressure of internal perfusion. Therefore, four different effects can be recorded for a given concentration of agonist by combining the variables related to the route of perfusion (external or internal) and type of muscle (longitudinal or circular). In addition, antagonism or synergism can be studied by simultaneously perfusing a second drug. Results can be expressed as single records or as mean concentration-response curves from which drug-receptor parameters can be directly or indirectly obtained. The importance of employing this method for the analysis of some less usual problems related to the mechanism of drug action is discussed.
详细描述了一种用于输精管外部和管腔双重灌注的装置。该灌注系统允许同时记录药物诱导的或器官环形和纵行平滑肌层的自发收缩。纵行（外部）层的等长收缩通过张力换能器记录。环形（内部）平滑肌层的收缩记录为内部灌注压力的变化。因此，通过组合与灌注途径（外部或内部）和肌肉类型（纵行或环形）相关的变量，可以记录给定浓度激动剂的四种不同效应。此外，可以通过同时灌注第二种药物来研究拮抗作用或协同作用。结果可以表示为单个记录或平均浓度-反应曲线，从中可以直接或间接获得药物-受体参数。讨论了采用这种方法分析一些与药物作用机制相关的不太常见问题的重要性。
