Kroplewski Maciej, Orzel-Gryglewska Jolanta, Nowacka Anita, Trojniar Weronika, Jurkowlaniec Edyta
Department of Animal Physiology, Gdańsk University, Gdańsk, Poland.
Acta Neurobiol Exp (Wars). 2010;70(3):261-70. doi: 10.55782/ane-2010-1797.
The nucleus reticularis pontis oralis (RPO) is a reticular structure important for the regulation of paradoxical sleep (PS). However, the data concerning the relation between the RPO and the main tonic indicator of PS, hippocampal theta rhythm, are contradictory: although electrical or cholinergic stimulation of the RPO evoked well-synchronized theta activity, the electrolytic lesion of the structure had no effect on theta. In our experiment, the effect of procaine injections into different parts of the RPO on the electrical activity of the hippocampus, as well as on tail pinch-elicited hippocampal theta rhythm was assessed in urethanized rats. Power spectral analysis was performed using a Fast Fourier Transform routine in 1-Hz and 3-Hz bands between 0.6 and 12 Hz frequency. We have found that unilateral procaine inactivation of neurons in the caudal part of the RPO blocked the sensory-elicited theta rhythm. The same injection into the rostral RPO either had no effect or evoked long-lasting episodes of theta rhythm without sensory stimulation. These results suggest functional diversity of the parts of the RPO in mechanisms underlying production of hippocampal theta.
脑桥嘴侧网状核（RPO）是一种对异相睡眠（PS）调节很重要的网状结构。然而，关于RPO与PS的主要紧张性指标——海马θ节律之间关系的数据相互矛盾：尽管对RPO进行电刺激或胆碱能刺激可诱发同步良好的θ活动，但该结构的电解损伤对θ节律并无影响。在我们的实验中，评估了向RPO不同部位注射普鲁卡因对海马电活动以及对夹尾诱发的海马θ节律的影响，实验对象为用乌拉坦麻醉的大鼠。使用快速傅里叶变换程序在0.6至12赫兹频率之间的1赫兹和3赫兹频段进行功率谱分析。我们发现，单侧将RPO尾部的神经元用普鲁卡因使其失活，会阻断感觉诱发的θ节律。向RPO头端进行同样的注射要么没有效果，要么在无感觉刺激的情况下诱发长时间的θ节律发作。这些结果表明，RPO各部分在海马θ产生机制中具有功能多样性。
