Department of Neurology, Sheng Jing Hospital of China Medical University, Shenyang, 110001, China.
J Mol Neurosci. 2011 Mar;43(3):370-5. doi: 10.1007/s12031-010-9453-7. Epub 2010 Sep 25.
The apolipoprotein M (ApoM) gene is critical in the formation of pre-β-high-density lipoprotein (HDL) and cholesterol efflux to HDL. In this case and control study, 314 ischemic stroke patients and 389 healthy controls were analyzed for three ApoM gene single-nucleotide polymorphisms (SNPs), i.e., C-1065A, T-855C, and T-778C, using a SNaPshot Multiplex sequencing assay. The genotype and allele frequencies of the T-855C were similar in both ischemic stroke patients and the controls. But the frequency of the TC genotype, the C allele of T-778C, and the A allele of the C-1065A SNPs in ischemic stroke patients was significantly higher than that of the healthy controls. After adjusting for confounding risk factors (such as hypertension, diabetes, tobacco smoking, and alcohol consumption), the ApoM gene TC genotype, C allele of T-778C, and A allele of C-1065A were associated with a risk of ischemic stroke. Moreover, plasma levels of total cholesterol were significantly higher in patients with CC or CT genotypes of T-778C than those with TT genotype in the controls. The current data demonstrated that ApoM T-778 C and C-1065A SNPs were associated with increased risk of ischemic stroke in this Han Chinese population.
载脂蛋白 M (ApoM) 基因对于前-β 高密度脂蛋白 (HDL) 的形成和胆固醇向 HDL 的流出至关重要。在这项病例对照研究中，分析了 314 名缺血性脑卒中患者和 389 名健康对照者的三个 ApoM 基因单核苷酸多态性 (SNP)，即 C-1065A、T-855C 和 T-778C，使用 SNaPshot 多重测序测定法。T-855C 的基因型和等位基因频率在缺血性脑卒中患者和对照组中相似。但缺血性脑卒中患者 TC 基因型、T-778C 的 C 等位基因和 C-1065A SNP 的 A 等位基因的频率明显高于健康对照组。在调整混杂风险因素（如高血压、糖尿病、吸烟和饮酒）后，ApoM 基因 TC 基因型、T-778C 的 C 等位基因和 C-1065A 的 A 等位基因与缺血性脑卒中的风险相关。此外，与 TT 基因型相比，T-778C 的 CC 或 CT 基因型患者的总胆固醇水平明显升高。目前的数据表明，在本汉族人群中，ApoM T-778C 和 C-1065A SNP 与缺血性脑卒中风险增加相关。
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