Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
J Mol Neurosci. 2012 Jul;47(3):458-64. doi: 10.1007/s12031-012-9721-9. Epub 2012 Feb 19.
Arachidonate 12/15-lipoxygenase (12/15-LOX) is a member of the lipid peroxidizing enzyme family and plays a major role in atherosclerosis. The present study aimed to evaluate whether or not the ALOX15 gene polymorphism is involved in ischemic stroke in northern Chinese Han population. Participants in a case-control study included 396 patients (239 males, 157 females) with ischemic stroke and 360 healthy subjects (211 males, 149 females). The rs7217186 polymorphism of the ALOX15 gene was analyzed by polymerase chain reaction and restriction fragment length polymorphism, while the rs2619112 polymorphism of the ALOX15 gene was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The frequencies of the CC genotype and the C allele of rs7217186 were higher in participants with ischemic stroke than in the control group [P = 0.014 in males, P = 0.007 in atherosclerosis ischemic stroke (ACI)]. The frequencies of the AA genotype and the A allele of rs2619112 were higher in participants with ischemic stroke than in the control group (P = 0.011 in males, P = 0.015 in ACI). Multiple logistic regression analysis revealed the significance of rs7217186 and rs2619112 in males and in ACI after adjustment for confounding factors. The rs7217186 and rs2619112 polymorphisms of the ALOX15 gene were associated with ischemic stroke in males and in patients with ACI in northern Chinese Han population.
花生四烯酸 12/15-脂加氧酶（12/15-LOX）是脂质过氧化酶家族的一员，在动脉粥样硬化中起主要作用。本研究旨在评估 ALOX15 基因多态性是否与中国北方汉族人群的缺血性卒中有关。病例对照研究的参与者包括 396 例缺血性卒中患者（239 名男性，157 名女性）和 360 名健康对照者（211 名男性，149 名女性）。通过聚合酶链反应和限制性片段长度多态性分析 ALOX15 基因的 rs7217186 多态性，通过基质辅助激光解吸/电离飞行时间质谱分析 ALOX15 基因的 rs2619112 多态性。rs7217186 的 CC 基因型和 C 等位基因在缺血性卒中患者中的频率高于对照组[男性 P=0.014，动脉粥样硬化性缺血性卒中（ACI） P=0.007]。rs2619112 的 AA 基因型和 A 等位基因在缺血性卒中患者中的频率高于对照组（男性 P=0.011，ACI P=0.015）。多因素 logistic 回归分析显示，在调整混杂因素后，rs7217186 和 rs2619112 在男性和 ACI 中具有统计学意义。ALOX15 基因的 rs7217186 和 rs2619112 多态性与中国北方汉族男性缺血性卒中及 ACI 患者有关。
