新生仔猪脑缺氧缺血后细胞凋亡活性的早期区域性反应。
Early regional response of apoptotic activity in newborn piglet brain following hypoxia and ischemia.
机构信息
Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
出版信息
Neurochem Res. 2011 Jan;36(1):83-92. doi: 10.1007/s11064-010-0267-3. Epub 2010 Sep 26.
Abstract
Responses of selected neuroregulatory proteins that promote (Caspase 3 and Bax) or inhibit (Bcl-2, high Bcl-2/Bax ratio) apoptotic cell death were measured in the brain of piglets subjected to precisely controlled hypoxic and ischemic insults: 1 h hypoxia (decreasing FiO₂ from 21 to 6%) or ischemia (ligation of carotid arteries and hemorrhage), followed by 0, 2 and 4 h recovery with 21% FiO₂. Protein expression was measured in cortex, hippocampus and striatum by Western blot. There were no significant differences in expression of Caspase-3 between sham operated, hypoxic and ischemic groups. There were significant regional differences in expression of Bcl-2 and Bax in response to hypoxia and ischemia. The changes in Bcl-2/Bax ratio were similar for hypoxia and ischemia except for striatum at zero time recovery, with ischemia giving lower ratios than hypoxia. The Bcl-2/Bax ratio was also lower for the striatum than for the other regions of the brain, suggesting this region is the more susceptible to apoptotic injury.
摘要
在接受精确控制的缺氧和缺血性损伤的仔猪的大脑中，测量了促进（Caspase 3 和 Bax）或抑制（Bcl-2、高 Bcl-2/Bax 比值）细胞凋亡的选定神经调节蛋白的反应：1 小时缺氧（将 FiO₂从 21%降低至 6%）或缺血（颈动脉结扎和出血），然后在 21% FiO₂下恢复 0、2 和 4 小时。通过 Western blot 在皮质、海马体和纹状体中测量蛋白表达。在假手术、缺氧和缺血组之间 Caspase-3 的表达没有显著差异。Bcl-2 和 Bax 的表达在缺氧和缺血反应中存在显著的区域差异。Bcl-2/Bax 比值的变化对于缺氧和缺血是相似的，除了在零时间恢复的纹状体，缺血的比值低于缺氧。Bcl-2/Bax 比值对于纹状体也低于大脑的其他区域，表明该区域更容易受到凋亡损伤。
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