Clinic of Neurology am Marienplatz, Munich, Germany.
J Med Virol. 2010 Nov;82(11):1917-20. doi: 10.1002/jmv.21904.
Herpes simplex virus type-1 latency and CD8+ T-cell occurrence were investigated in the trigeminal, geniculate, and vestibular ganglia from seven deceased humans. The HSV-1 "latency-associated transcript" was assessed by in situ hybridization and quantitative RT-PCR. Infiltration of CD8+ T cell was detected by immunohistochemistry and quantitative RT-PCR. The data show that HSV-1 latency and CD8+ T-cell infiltration are not solely confined to the trigeminal ganglia but can also occur in other cranial ganglia along the neuroaxis. However, the HSV-1 latency transcripts in the geniculate and vestibular ganglia were expressed at a very low level. The difference in CD8 transcript levels among HSV-1 latently infected trigeminal ganglia, geniculate, and vestibular ganglia was less conspicuous. Colocalization of latent HSV-1 and CD8+ T cells in geniculate and vestibular ganglia supports further the hypothesis that HSV-1 reactivation is possible in these ganglia and is the cause of Bell's palsy and vestibular neuritis.
研究了七种已故人类的三叉神经、膝状神经节和前庭神经节中单纯疱疹病毒 1 型（HSV-1）潜伏和 CD8+T 细胞的出现情况。通过原位杂交和定量 RT-PCR 评估 HSV-1“潜伏相关转录物”。通过免疫组织化学和定量 RT-PCR 检测 CD8+T 细胞浸润。数据表明，HSV-1 潜伏和 CD8+T 细胞浸润不仅局限于三叉神经节，还可以发生在沿神经轴的其他颅神经节中。然而，膝状神经节和前庭神经节中的 HSV-1 潜伏转录本表达水平非常低。HSV-1 潜伏感染的三叉神经节、膝状神经节和前庭神经节中 CD8 转录本水平的差异不明显。潜伏 HSV-1 和 CD8+T 细胞在膝状神经节和前庭神经节中的共定位进一步支持 HSV-1 再激活可能发生在这些神经节中，并导致贝尔氏麻痹和前庭神经炎的假说。
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