Hüfner Katharina, Derfuss Tobias, Herberger Simone, Sunami Kishiko, Russell Steven, Sinicina Inga, Arbusow Viktor, Strupp Michael, Brandt Thomas, Theil Diethilde
Department of Neurology, Klinikum Grosshadern, Ludwig-Maximilians University, Munich, Germany.
J Neuropathol Exp Neurol. 2006 Oct;65(10):1022-30. doi: 10.1097/01.jnen.0000235852.92963.bf.
The immune response to latent herpesvirus infections was compared in human trigeminal ganglia (TG) and dorsal root ganglia (DRG) of 15 dead individuals. On the basis of our previous findings, we hypothesized that T-cells would be attracted to sensory neurons latently infected with herpes simplex virus type 1 (HSV-1), but not to those harboring latent varicella zoster virus (VZV). We showed that the TG contain a positive hybridization signal for HSV-1 latency-associated transcript (LAT), whereas the DRG from the same individuals lack detectable LAT. In contrast, immunohistochemistry revealed that latent VZV protein 62 stained positive in the vast majority of all tested TG and DRG. T-cell infiltrates prominently surrounded individual neurons in the TG but not in the DRG. TaqMan polymerase chain reaction also showed higher expression of CD8 and RANTES transcripts in the TG versus DRG. Only the infiltrates in the TG, but not in the DRG, produced RANTES at the protein level. Because it has been shown that RANTES protein is produced only after T-cell receptor stimulation, we assume that T-cell infiltration is associated with antigen recognition in the TG but not in the DRG.
对15名死者的人类三叉神经节（TG）和背根神经节（DRG）中潜伏性疱疹病毒感染的免疫反应进行了比较。基于我们之前的研究结果，我们假设T细胞会被潜伏感染1型单纯疱疹病毒（HSV-1）的感觉神经元吸引，但不会被携带潜伏性水痘带状疱疹病毒（VZV）的神经元吸引。我们发现TG中存在HSV-1潜伏相关转录本（LAT）的阳性杂交信号，而同一患者的DRG中未检测到LAT。相反，免疫组织化学显示，在所有测试的TG和DRG中，绝大多数潜伏性VZV蛋白62呈阳性染色。T细胞浸润显著地围绕着TG中的单个神经元，但DRG中没有。TaqMan聚合酶链反应也显示，与DRG相比，TG中CD8和趋化因子调节激活正常T细胞表达和分泌因子（RANTES）转录本的表达更高。只有TG中的浸润细胞，而不是DRG中的浸润细胞，在蛋白质水平上产生RANTES。因为已经表明RANTES蛋白仅在T细胞受体刺激后产生，所以我们认为T细胞浸润与TG中的抗原识别有关，而与DRG中的无关。
