Center for Advanced Research in Indian System of Medicine, SASTRA University, Thirumalaisamudram, Thanjavur-613402, Tamilnadu, India.
Curr Pharm Biotechnol. 2010 Dec;11(8):887-94. doi: 10.2174/138920110793262033.
With recent advances in nutrition sciences, natural products and health-promoting foods have received extensive attention from both health professionals and the common population. The flavonoid rich fraction (FRF) of Dioscorea bulbifera Linn. has a strong free radical scavenging activity. FRF (150 mg/kg) when intervened for a period of 35 days prior to isoproterenol (ISO) challenge to rats maintained the creatine kinase - MB (CK-MB) activity in serum without elevation. Alterations in the antioxidant status in the mitochondria were recognized in the heart tissue of ISO induced rats. ISO induced rats pretreated with FRF (150 mg/kg) ameliorated the lipid peroxidation and thereby enhanced the antioxidant status as evidenced by the increase in the reduced glutathione (GSH) content and the activity of antioxidant enzymes. Moreover, the tricarboxylic acid cycle enzymes such isocitrate dehydrogenase (ICDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH) and α-ketoglutarate dehydrogenase (α-KGDH), which were found decreased in the ISO induced rats showed an enhanced activity in FRF (150 mg/kg) pretreated rats. The activity of NADH dehydrogenase and cytochrome-C-oxidase the enzymes, which transfer the electron in the electron transport chain (ETC) was also increased significantly (p<0.05) in FRF (150 mg/kg) pretreated rats, when compared with ISO induced rats. These results suggest the cardioprotective effect of FRF of Dioscorea bulbifera Linn. in ISO induced MI by attenuating the lipid peroxidation by scavenging free radicals and modulating the energy producing mitochondrial enzymes.
随着营养科学的最新进展，天然产物和促进健康的食品受到了健康专业人员和普通民众的广泛关注。盾叶薯蓣富含黄酮的部分（FRF）具有很强的清除自由基活性。FRF（150mg/kg）在异丙肾上腺素（ISO）挑战前干预 35 天，可维持血清肌酸激酶-MB（CK-MB）活性不升高。在 ISO 诱导的大鼠心脏组织中发现线粒体抗氧化状态发生改变。用 FRF（150mg/kg）预处理 ISO 诱导的大鼠可改善脂质过氧化作用，从而增强抗氧化状态，这表现为还原型谷胱甘肽（GSH）含量增加和抗氧化酶活性增强。此外，三羧酸循环酶（如异柠檬酸脱氢酶（ICDH）、琥珀酸脱氢酶（SDH）、苹果酸脱氢酶（MDH）和α-酮戊二酸脱氢酶（α-KGDH））在 ISO 诱导的大鼠中发现减少，而在 FRF（150mg/kg）预处理的大鼠中显示出增强的活性。在 FRF（150mg/kg）预处理的大鼠中，电子传递链（ETC）中传递电子的 NADH 脱氢酶和细胞色素-C-氧化酶的活性也显著增加（p<0.05），与 ISO 诱导的大鼠相比。这些结果表明，盾叶薯蓣 FRF 通过清除自由基和调节产生能量的线粒体酶来减轻脂质过氧化，从而对 ISO 诱导的心肌梗塞具有心脏保护作用。
