约旦志愿者中N-乙酰基转移酶-2（NAT2）的基因型频率

N-Acetyltransferase-2 (NAT2) genotype frequency among Jordanian volunteers.

作者信息

Jarrar Y B, Ismail S, Irshaid Y M

机构信息

Departments of Pharmacology, University of Jordan, Amman, Jordan.

出版信息

Int J Clin Pharmacol Ther. 2010 Oct;48(10):688-94. doi: 10.5414/cpp48688.

DOI:10.5414/cpp48688

PMID:20875375

Abstract

OBJECTIVE

To determine the frequency of major NAT2 alleles and genotypes among the Jordanian population.

METHODS

DNA samples from 150 healthy Jordanian volunteers were analyzed by polymerase chain reaction followed by restriction fragment length polymorphism assays (PCR-RFLP) to determine the frequency of four major alleles: NAT24, NAT25, NAT26 and NAT27.

RESULTS

The frequency (95% confidence interval) of NAT24, NAT25, NAT26 and NAT27 alleles was 0.247 (0.198 - 0.296), 0.373 (0.318 - 0.428), 0.347. 0.293 - 0.401) and 0.033 (0.013 - 0.053), respectively. The most prevalent genotypes are those which encode slow acetylation phenotype. Around 58.7%, 33.3% and 8% of volunteers carried the slow, the intermediate and the fast-encoding genotypes, respectively.

CONCLUSION

NAT2 genotype profile among Jordanians is similar to Caucasians. However, NAT2*6 allele is slightly high in comparison with Arab Middle Eastern populations.

摘要

目的

确定约旦人群中主要NAT2等位基因和基因型的频率。

方法

采用聚合酶链反应（PCR）及限制性片段长度多态性分析（PCR-RFLP），对150名健康约旦志愿者的DNA样本进行分析，以确定4种主要等位基因：NAT24、NAT25、NAT26和NAT27的频率。

结果

NAT24、NAT25、NAT26和NAT27等位基因的频率（95%置信区间）分别为0.247（0.198 - 0.296）、0.373（0.318 - 0.428）、0.347（0.293 - 0.401）和0.033（0.013 - 0.053）。最常见的基因型是编码慢乙酰化表型的基因型。分别约有58.7%、33.3%和8%的志愿者携带慢、中、快编码基因型。