Department of Water and Soil Analysis, Faculty of Chemistry, Adam Mickiewicz University, Drzymały 24, 60-613 Poznań, Poland.
Talanta. 2010 Oct 15;82(5):1763-9. doi: 10.1016/j.talanta.2010.07.066. Epub 2010 Aug 3.
The study presents a new analytical method for speciation analysis in fractionation of aluminium fluoride complexes and free Al(3+) in soil samples. Aluminium speciation was studied in model solutions and soil extract samples by means of high performance ion chromatography (HPIC) with UV-VIS detection using post-column reaction with tiron for the separation and detection of aluminium fluoride complex and Al(3+) forms during one analysis. The paper presents particular stages of the chromatographic process optimization involving selecting the appropriate eluent strength, type of elution or concentration and quantity of derivatization reagent. HPIC was performed on a bifunctional analytical column Dionex IonPac CS5A. The use of gradient elution and the eluents A: 1M NH(4)Cl and B: water acidified to pH of eluent phase, enabled full separation of fluoride aluminium forms as AlF(2)(+), AlF(3)(0), AlF(4)(-) (first signal), AlF(2+) (second signal) and form Al(3+) in a single analytical procedure. The proposed new method HPIC-UVVIS was applied successfully in the quantitative and qualitative analysis of soil samples.
该研究提出了一种新的分析方法，用于在氟化铝络合物和土壤样品中游离 Al(3+)的分级分析中进行形态分析。通过使用高效离子色谱 (HPIC) 与 UV-VIS 检测，通过与钛铁试剂的柱后反应，在一次分析中分离和检测氟化铝络合物和 Al(3+)形式，在模型溶液和土壤提取样品中研究了铝的形态。本文介绍了色谱过程优化的特定阶段，包括选择适当的洗脱液强度、洗脱类型或浓度以及衍生试剂的量。HPIC 在 Dionex IonPac CS5A 双功能分析柱上进行。使用梯度洗脱和洗脱液 A: 1M NH(4)Cl 和 B: 水酸化至洗脱相 pH，可在单个分析程序中完全分离氟铝形态，如 AlF(2)(+)、AlF(3)(0)、AlF(4)(-)（第一个信号）、AlF(2+)（第二个信号）和 Al(3+)形式。所提出的新方法 HPIC-UVVIS 成功应用于土壤样品的定量和定性分析。
