Department of Physics, National Taiwan University, Taipei 106, Taiwan.
Biomaterials. 2010 Dec;31(36):9415-21. doi: 10.1016/j.biomaterials.2010.08.055. Epub 2010 Sep 26.
Using excitation polarization-resolved second harmonic generation (SHG) microscopy, we measured SHG intensity as a function of the excitation polarization angle for type I and type II collagens. We determined the second order susceptibility (χ((2))) tensor ratios of type I and II collagens at each pixel, and displayed the results as images. We found that the χ((2)) tensor ratios can be used to distinguish the two types of collagen. In particular, we obtained χ(zzz)/χ(zxx) = 1.40 ± 0.04 and χ(xzx)/χ(zxx) = 0.53 ± 0.10 for type I collagen from rat tail tendon, and χ(zzz)/χ(zxx) = 1.14 ± 0.09 and χ(xzx)/χ(zxx) = 0.29 ± 0.11 for type II collagen from rat trachea cartilage. We also applied this methodology on the label-free imaging of engineered cartilage tissue which produces type I and II collagen simultaneously. By displaying the χ((2)) tensor ratios in the image format, the variation in the χ((2)) tensor ratios can be used as a contrast mechanism for distinguishing type I and II collagens.
利用激发偏极化分辨二次谐波产生（SHG）显微镜，我们测量了 I 型和 II 型胶原的激发偏光角度的 SHG 强度。我们在每个像素处确定了 I 型和 II 型胶原的二阶极化率张量比，并将结果显示为图像。我们发现，χ((2))张量比可用于区分两种类型的胶原。具体而言，我们从大鼠尾腱中获得了 I 型胶原的 χ(zzz)/χ(zxx)=1.40±0.04 和 χ(xzx)/χ(zxx)=0.53±0.10，从大鼠气管软骨中获得了 II 型胶原的 χ(zzz)/χ(zxx)=1.14±0.09 和 χ(xzx)/χ(zxx)=0.29±0.11。我们还将此方法应用于同时产生 I 型和 II 型胶原的工程化软骨组织的无标记成像。通过以图像格式显示 χ((2))张量比，可以将 χ((2))张量比的变化用作区分 I 型和 II 型胶原的对比机制。
