Department of Physics, University of Toronto, Ontario, Canada.
Department of Chemical and Physical Sciences, University of Toronto Mississauga, Ontario, Canada.
J Biophotonics. 2019 Jan;12(1):e201800241. doi: 10.1002/jbio.201800241. Epub 2018 Oct 14.
Polarization-dependent second-harmonic generation (P-SHG) microscopy is used to characterize molecular nonlinear optical properties of collagen and determine a three-dimensional (3D) orientation map of collagen fibers within a pig tendon. C symmetry is used to determine the nonlinear susceptibility tensor components ratios in the molecular frame of reference and , where the latter is a newly extracted parameter from the P-SHG images and is related to the chiral structure of collagen. The is observed for collagen fibers tilted out of the image plane, and can have positive or negative values, revealing the relative polarity of collagen fibers within the tissue. The P-SHG imaging was performed using a linear polarization-in polarization-out (PIPO) method on thin sections of pig tendon cut at different angles. The nonlinear chiral properties of collagen can be used to construct the 3D organization of collagen in the tissue and determine the orientation-independent molecular susceptibility ratios of collagen fibers in the molecular frame of reference.
偏振相关二次谐波产生（P-SHG）显微镜用于表征胶原的分子非线性光学性质，并确定猪肌腱内胶原纤维的三维（3D）取向图。C 对称性用于确定分子参考系中的非线性有效张量分量比 ，其中后者是从 P-SHG 图像中提取的新参数，与胶原的手性结构有关。对于偏离图像平面的胶原纤维，观察到 ，其值可以为正或负，揭示了组织内胶原纤维的相对极性。P-SHG 成像使用线性偏振入-偏振出（PIPO）方法在不同角度切割的猪肌腱薄片上进行。胶原的非线性手性特性可用于构建组织中胶原的 3D 组织，并确定分子参考系中胶原纤维的与取向无关的分子有效张量分量比。
