Department of Plant Biology, University of Alcalá, 28871-Alcalá de Henares, Madrid, Spain.
Mol Phylogenet Evol. 2010 Dec;57(3):1323-8. doi: 10.1016/j.ympev.2010.09.014. Epub 2010 Sep 27.
The scarcity of universally applied molecular markers for algae has resulted in the development of multiple, independent and not easily comparable systems. The goal of this work is to increase the number of available molecular markers and to generate easily comparable systems. Thereby, we have designed a primer pair capable of amplifying a broad range of organisms: Cyanobacteria, Chlorophyta, Chlorarachniophyta, Cryptophyta, Euglenida, Glaucophyta, Rhodophyta, Stramenopiles and Streptophyta including plants. This primer pair can amplify a portion of the 23S rRNA gene with sufficient variability to identify reference material form collections across a broad range of taxa and perform phylogenetic studies alongside other available markers.
藻类普遍适用的分子标记物的稀缺性导致了多种独立且不易比较的系统的发展。这项工作的目的是增加可用的分子标记物的数量，并生成易于比较的系统。为此，我们设计了一对引物，能够扩增广泛的生物：蓝藻、绿藻、绿藻门、隐藻门、眼虫藻、裸藻门、红藻门、不等鞭毛门和有胚植物门，包括植物。该引物对可以扩增 23S rRNA 基因的一部分，其变异性足以鉴定来自广泛分类群的收藏材料，并与其他可用标记物一起进行系统发育研究。
