Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, PO Box 110910, Gainesville, Florida 32611-0910, USA.
Reproduction. 2010 Dec;140(6):815-26. doi: 10.1530/REP-10-0190. Epub 2010 Sep 27.
The ability of oocytes to resume meiosis, become fertilized, and generate viable pregnancies is controlled during folliculogenesis by several endocrine and paracrine factors. The aim of this work is to determine whether fibroblast growth factor 10 (FGF10) is an oocyte competent factor. Transcripts for each of the four FGF receptor types (FGFR) were present in cumulus and oocytes after their extraction from the follicles. FGFR1 transcripts predominated in cumulus cells whereas FGFR2 was most abundant in oocytes. Exposing the cumulus-oocyte complexes to FGF10 during in vitro maturation did not affect cleavage rates, but increases (P<0.05) in the percentage of embryos at the 8-16-cell stage on day 3 and at the blastocyst stage on day 7, which were evident in FGF10-supplemented oocytes. The progression of oocytes through meiosis and cumulus expansion was increased (P<0.05) by FGF10. The importance of the endogenous sources of FGFs was examined by adding anti-FGF10 IgG during oocyte maturation. Blocking endogenous FGF10 activity decreased (P<0.05) the percentage of oocytes developing into blastocysts and limited (P<0.05) cumulus expansion. Expression profiles of putative cumulus and oocyte competency markers were examined for their involvement in FGF10-mediated responses. FGF10 influenced the expression of CTSB and SPRY2 in cumulus cells and BMP15 in oocytes. In summary, this work provides new insight into the importance of FGFRs and locally derived FGF10 during oocyte maturation in cattle. Its subsequent impact on in vitro embryo development implicates it as a noteworthy oocyte competent factor.
卵母细胞恢复减数分裂、受精和产生可存活妊娠的能力，在卵泡发生过程中受几种内分泌和旁分泌因子的控制。本研究旨在确定成纤维细胞生长因子 10（FGF10）是否是卵母细胞的一种有能力的因子。在从卵泡中提取卵丘和卵母细胞后，四种 FGF 受体类型（FGFR）的转录本都存在于其中。在卵丘细胞中 FGFR1 转录本占主导地位，而 FGFR2 在卵母细胞中最为丰富。在体外成熟过程中，将卵丘-卵母细胞复合物暴露于 FGF10 中，不会影响卵裂率，但会增加（P<0.05）第 3 天处于 8-16 细胞阶段和第 7 天处于囊胚阶段的胚胎百分比，这在补充 FGF10 的卵母细胞中很明显。FGF10 增加了卵母细胞通过减数分裂和卵丘扩展的进展（P<0.05）。通过在卵母细胞成熟过程中添加抗 FGF10 IgG 来检查内源性 FGFs 来源的重要性。阻断内源性 FGF10 活性降低了（P<0.05）卵母细胞发育成囊胚的百分比，并限制了（P<0.05）卵丘的扩展。检查了推定的卵丘和卵母细胞能力标志物的表达谱，以研究它们在 FGF10 介导的反应中的作用。FGF10 影响卵丘细胞中 CTSB 和 SPRY2 的表达以及卵母细胞中 BMP15 的表达。总之，这项工作为 FGFRs 和牛卵母细胞成熟过程中局部来源的 FGF10 的重要性提供了新的见解。它随后对体外胚胎发育的影响表明它是一种值得注意的卵母细胞有能力的因子。
