School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
J Clin Pathol. 2010 Oct;63(10):894-9. doi: 10.1136/jcp.2010.078964.
The assessment of malignant potential of oral submucous fibrosis grades vis-à-vis their progression towards malignancy is associated with expression of possible multiple molecular markers.
To analyse p63, E-cadherin and CD105 expression in this premalignant pathosis with a view to unravel and understand the expression of these molecules as markers.
The oral mucosal biopsies (normal, oral submucous fibrosis with and without dysplasia) were studied with routine H&E, and by immunohistochemistry for p63, E-cadherin and CD105 expression. p63 was assessed as percentage of positive nuclei. E-cadherin expression was estimated through (i) distance between basement membrane and E-cadherin expression initiation point, (ii) ratio between epithelial thickness and epithelial thickness displaying E-cadherin, and (iii) E-cadherin intensity variation along the expression path. CD105 expression was assessed qualitatively.
The p63+ cells were highest in severely dysplastic tissues followed by other dysplastic grades, normal oral mucosa and non-dysplastic conditions. However, the p63+ cells displayed the feature of progressive maturation only in normal mucosa. There was a loss of membranous E-cadherin in basal layers of all diseased conditions; it was highest in severe dysplasia. There was significant variation (p<0.0001) in E-cadherin intensity within and between the tissues (normal and diseased). CD105 expression increased abruptly in dysplasia.
The malignant potential of this pre-cancerous condition is likely to be correlated with an increase in p63 and CD105 expression and a concomitant loss of membranous E-cadherin. This may lead to marker identification through greater validation.
评估口腔黏膜下纤维性变的恶性潜能及其向恶性转化的程度与可能的多种分子标志物的表达有关。
分析口腔黏膜下纤维性变中 p63、E-钙黏蛋白和 CD105 的表达，以揭示和理解这些分子作为标志物的表达。
对口腔黏膜活检标本（正常、有和无异型增生的口腔黏膜下纤维性变）进行常规 H&E 染色，并通过免疫组织化学方法检测 p63、E-钙黏蛋白和 CD105 的表达。p63 的阳性核百分比进行评估。E-钙黏蛋白的表达通过（i）基底膜与 E-钙黏蛋白表达起始点之间的距离、（ii）上皮厚度与显示 E-钙黏蛋白的上皮厚度之比，以及（iii）沿表达途径的 E-钙黏蛋白强度变化来评估。CD105 的表达进行定性评估。
在重度异型增生组织中 p63+细胞最多，其次是其他异型增生分级、正常口腔黏膜和非异型增生组织。然而，p63+细胞仅在正常黏膜中表现出逐渐成熟的特征。在所有病变组织中，基底细胞层均缺失膜性 E-钙黏蛋白；在重度异型增生中缺失最多。E-钙黏蛋白强度在组织内和组织间（正常和病变）均有显著差异（p<0.0001）。在异型增生中 CD105 的表达突然增加。
这种癌前病变的恶性潜能可能与 p63 和 CD105 表达的增加以及膜性 E-钙黏蛋白的丧失相关。这可能通过进一步验证来识别标志物。
