School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
Biotechnol J. 2012 May;7(5):602-7. doi: 10.1002/biot.201100013. Epub 2011 Oct 26.
Precise histopathological localization of E-cadherin and p63 is of immense importance in understanding the integrity of oral mucosal stratified epithelium in normal and diseased conditions. Necessarily immunohistochemical imaging should have minimum bleaching impact on the dyes and ability to produce clear and crisp images. Here ApoTome provides an alternative with metal halide light source and structured illumination under the assistance of grids, along with integrated image processing modality to generate crisp images with digital interface. The current study demonstrates the applicability of such microscopic system in capturing fluorescence images of immunohistochemical sections of normal and precancerous biopsies in respect to the expression of p63 and E-cadherin in the epithelial cells. The ApoTome images localize the nuclear and membranous expressions of p63 and E-cadherin, respectively, with remarkable specificity. The findings on E-cadherin expression have enormous diagnostic significance as these images clearly differentiate the early and advanced stages of oral submucous fibrosis based on their cytoplasmic and membranous location. Thus, this study clearly depicts a remarkable performance of ApoTome with diagnostic significance.
精确的组织病理学定位 E-钙黏蛋白和 p63 对于理解口腔黏膜复层上皮在正常和疾病状态下的完整性具有重要意义。免疫组织化学成像必然对染料的漂白影响最小，并且能够产生清晰、鲜明的图像。在这里，ApoTome 在网格的辅助下，提供了一种替代方法，使用金属卤化物光源和结构照明，并结合集成的图像处理方式，生成具有数字接口的鲜明图像。本研究证明了这种显微镜系统在捕获正常和癌前活检的免疫组织化学切片的荧光图像方面的适用性，涉及上皮细胞中 p63 和 E-钙黏蛋白的表达。ApoTome 图像分别定位 p63 和 E-钙黏蛋白的核和膜表达，具有显著的特异性。E-钙黏蛋白表达的发现具有巨大的诊断意义，因为这些图像根据其细胞质和膜定位清楚地区分了口腔黏膜下纤维性变的早期和晚期阶段。因此，本研究清楚地描绘了 ApoTome 具有诊断意义的卓越性能。
