School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, People's Republic of China.
Biomed Mater. 2010 Oct;5(5):054115. doi: 10.1088/1748-6041/5/5/054115. Epub 2010 Sep 28.
Micro-patterns with deposited calcium/phosphorus (Ca/P) elements on a pure titanium (Ti) surface were synthesized in 2.5 x simulated body fluid (SBF) by femtosecond laser ablation, and their bioactivities were evaluated by co-culture with osteoblasts. Scanning electron microscope observation showed that a three-order roughness micro-pattern, which was composed of grooves, islands, holes and sub-micro-particles, was obtained, and the size of holes and islands was increased from 0.5 µm and 2 µm to 5 µm and 6 µm, respectively, gradually with the increase of laser fluence from 3.3 J cm(-2) to 12.5 J cm(-2). Irradiation of femtosecond lasers promoted the deposition of Ca/P on the surface of a Ti plate. The osteoblasts grew faster and extended more slender filopodia on the femtosecond laser-ablated surface than on a sand blast followed by an acid surface, which demonstrated that the micro-patterns with deposited Ca/P on the surface were favorable for osteoblasts' growth.
在 2.5×模拟体液（SBF）中，通过飞秒激光烧蚀在纯钛（Ti）表面沉积钙/磷（Ca/P）元素，合成具有微图案。通过与成骨细胞共培养来评估其生物活性。扫描电子显微镜观察表明，获得了由凹槽、岛、孔和亚微米颗粒组成的三阶粗糙度微图案，并且随着激光能量密度从 3.3 J/cm²增加到 12.5 J/cm²，孔和岛的尺寸分别从 0.5 µm 和 2 µm逐渐增加到 5 µm 和 6 µm。飞秒激光辐照促进了 Ca/P 在 Ti 板表面的沉积。成骨细胞在飞秒激光烧蚀表面上比在喷砂酸蚀表面上生长更快，并且延伸出更细长的丝状伪足，这表明表面沉积 Ca/P 的微图案有利于成骨细胞的生长。
