利用微弧氧化法对钛表面进行钙磷离子修饰及其对成骨细胞黏附的影响
[Modification of titanium surface with calcium and phosphorus ions using micro-arc oxidation and its effect on osteoblast attachment].
作者信息
Ma Chu-fan, Li Dong-mei, Li He-jun, Jiang Bai-ling, Zhang Li-jun
机构信息
Institute of Material Science and Material Engineering, Northwestern Polytechnical University, Xi'an 710073, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2005 Jan;25(1):62-5.
OBJECTIVE
To study the method for modifying titanium surface with calcium and phosphorus ions using micro-arc oxidation technique and observe osteoblast attachment to the modified surface.
METHODS
TA(2) titanium discs were treated with micro-arc oxidation in electrolyte solution containing Ca(2+) and PO(4)(3-). The influence of Ca(2+) and PO(4)(3-) concentrations in the solution and the electrical parameters of the micro-arc oxidization on the content of calcium and phosphorus ions incorporated into the surface of titanium was investigated using energy-dispersive X-ray (EDX) analysis. Scanning electron microscopy was employed for morphological observation of the ceramic coating on the metal surface. The binding strength of ceramic coating with titanium was tested by shear bonding experiment. MC-3T3-E1 osteoblast-like cells were then cultured on the treated surface of titanium discs to investigate the influence of the ceramic coating on osteoblast attachment.
RESULTS
Micro-arc oxidation treatment produced a layer of porous TiO(2) coating on the surface of titanium discs, with the average pore size of 2 to 10 mum. EDX analysis revealed that the ceramic coating contained Ca and P elements, whose content had close correlation with Ca(2+) and PO(4)(3-) concentrations in the electrolyte solution and voltage, duty cycle and frequency of micro-arc oxidation. The average bonding strength of the ceramic coating with titanium was 22+/-3 MPa, and TiO(2) coating promoted attachment and spread of osteoblast-like cells on the metal surface as demonstrated by cell culture.
CONCLUSIONS
Porous TiO(2) coating can be constructed on the surface of titanium using micro-arc oxidation, and Ca(2+) and PO(4)(3-) incorporated into the coating can improve the biocompatibility of titanium. The content of Ca(2+) and PO(4)(3-) in the coating can be modulated by adjusting the concentrations of Ca(2+) and PO(4)(3-) in the electrolyte solution and the electrical parameters of the micro-arc oxidation.
目的
研究采用微弧氧化技术用钙磷离子修饰钛表面的方法，并观察成骨细胞在修饰表面的附着情况。
方法
将TA(2)钛盘在含Ca(2+)和PO(4)(3-)的电解液中进行微弧氧化处理。采用能量色散X射线（EDX）分析研究溶液中Ca(2+)和PO(4)(3-)浓度以及微弧氧化的电参数对掺入钛表面的钙磷离子含量的影响。用扫描电子显微镜对金属表面的陶瓷涂层进行形态观察。通过剪切粘结实验测试陶瓷涂层与钛的结合强度。然后将MC-3T3-E1成骨样细胞接种在处理过的钛盘表面，研究陶瓷涂层对成骨细胞附着的影响。
结果
微弧氧化处理在钛盘表面产生了一层多孔的TiO(2)涂层，平均孔径为2至10μm。EDX分析表明，陶瓷涂层含有Ca和P元素，其含量与电解液中Ca(2+)和PO(4)(3-)的浓度以及微弧氧化的电压、占空比和频率密切相关。陶瓷涂层与钛的平均结合强度为22±3MPa，细胞培养结果表明TiO(2)涂层促进了成骨样细胞在金属表面的附着和铺展。
结论
采用微弧氧化可在钛表面构建多孔TiO(2)涂层，掺入涂层中的Ca(2+)和PO(4)(3-)可提高钛的生物相容性。通过调节电解液中Ca(2+)和PO(4)(3-) 的浓度以及微弧氧化的电参数，可以调控涂层中Ca(2+)和PO(4)(3-)的含量。
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