Feitosa Matheus Levi Tajra, Fadel Leandro, Beltrão-Braga Patrícia Cristina Baleeiro, Wenceslau Cristiane Valverde, Kerkis Irina, Kerkis Alexandre, Birgel Júnior Eduardo Harry, Martins João Flávio Panattoni, Martins Daniele dos Santos, Miglino Maria Angélica, Ambrósio Carlos Eduardo
Department of Surgery, Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo, Brazil.
Acta Cir Bras. 2010 Oct;25(5):416-22. doi: 10.1590/s0102-86502010000500006.
Evaluate the bone tissue recovery following transplantation of ovine mesenchymal stem cells (MSC) from bone marrow and human immature dental-pulp stem cells (hIDPSC) in ovine model of induced osteonecrosis of femoral head (ONFH).
Eight sheep were divided in three experimental groups. First group was composed by four animals with ONFH induced by ethanol through central decompression (CD), for control group without any treatment. The second and third group were compose by two animals, six weeks after ONFH induction received transplantation of heterologous ovine MSC (CD + oMSC), and hIDPSC (CD + hIDPSC), respectively. In both experiments the cells were transplanted without application of any type of immunosupression protocol.
Our data indicate that both cell types used in experiments were able to proliferate within injured site providing bone tissue recovery. The histological results obtained from CD+hIDPSC suggested that the bone regeneration in such animals was better than that observed in CD animals.
Mesenchymal stem cell transplant in induced ovine osteonecrosis of femoral head by central decompression technique is safe, and apparently favors bone regeneration of damaged tissues.
在绵羊股骨头缺血性坏死（ONFH）诱导模型中，评估移植来自骨髓的绵羊间充质干细胞（MSC）和人未成熟牙髓干细胞（hIDPSC）后的骨组织恢复情况。
将八只绵羊分为三个实验组。第一组由四只通过中心减压（CD）诱导产生ONFH的动物组成，作为未接受任何治疗的对照组。第二组和第三组各由两只动物组成，在诱导ONFH六周后，分别接受异源绵羊MSC（CD + oMSC）和hIDPSC（CD + hIDPSC）移植。在两个实验中，细胞移植均未应用任何类型的免疫抑制方案。
我们的数据表明，实验中使用的两种细胞类型均能够在损伤部位增殖，促进骨组织恢复。从CD + hIDPSC获得的组织学结果表明，此类动物的骨再生情况优于CD组动物。
通过中心减压技术将间充质干细胞移植到诱导的绵羊股骨头缺血性坏死中是安全的，并且显然有利于受损组织的骨再生。
