Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo 157-8535, Japan.
Int J Oncol. 2010 Nov;37(5):1279-88. doi: 10.3892/ijo_00000779.
It is hoped that the gangliosides contained in neuroblastomas (NBs) can be used as outcome predictors. We used liquid chromatography-tandem mass spectrometry (LC-MS) to analyze the gangliosides expressed in 11 NB cell lines. LC-MS analysis detected a number of gangliosides, including acetylated forms, with significantly higher sensitivity than conventional high-performance thin-layer chromatography analysis, and the results revealed that the expression profiles of the gangliosides GD1a, GD2, and acetylated GD2 differed according to the NB cell line. Hierarchical clustering based on the ganglioside expression profiles obtained by LC-MS analysis revealed that the NB cell lines could be classified into three types according to their expression of these three gangliosides: A-type characterized by high expression of GD1a and low or no expression of GD2/acetylated GD2, B-type characterized by low or no expression of GD1a and high expression of GD2/acetylated GD2, and AB-type characterized by expression of both GD1a and GD2/acetylated GD2. Interestingly, all three MYCN non-amplified cell lines were classified into the A-type. The classification was found to be correlated with mRNA expression of ganglioside synthase and neural-differentiation-related genes. The results of this study indicate that LC-MS analysis is useful as a tool for glycosphingolipid research on malignancies.
人们希望神经母细胞瘤（NBs）中含有的神经节苷脂可作为预后预测因子。我们使用液相色谱-串联质谱（LC-MS）分析了 11 种 NB 细胞系中表达的神经节苷脂。LC-MS 分析检测到许多神经节苷脂，包括乙酰化形式，其灵敏度明显高于传统的高效薄层色谱分析，结果表明神经节苷脂 GD1a、GD2 和乙酰化 GD2 的表达谱根据 NB 细胞系而有所不同。基于 LC-MS 分析获得的神经节苷脂表达谱进行的层次聚类显示，根据这三种神经节苷脂的表达，NB 细胞系可以分为三种类型：A型以 GD1a 高表达和 GD2/乙酰化 GD2 低表达或无表达为特征，B 型以 GD1a 低表达或无表达和 GD2/乙酰化 GD2 高表达为特征，AB 型以 GD1a 和 GD2/乙酰化 GD2 的表达为特征。有趣的是，所有三种 MYCN 非扩增细胞系均被归类为 A 型。该分类与神经节苷脂合酶和神经分化相关基因的 mRNA 表达相关。本研究结果表明，LC-MS 分析可用作恶性肿瘤糖脂研究的工具。
