神经母细胞瘤细胞谱系表型和模型概述。
An overview of neuroblastoma cell lineage phenotypes and models.
机构信息
Graduate Program in Health Sciences, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, Brazil.
Instituto de Pesquisa Pelé Pequeno Príncipe, Faculdades Pequeno Príncipe, Curitiba 80250-060, Brazil.
出版信息
Exp Biol Med (Maywood). 2020 Dec;245(18):1637-1647. doi: 10.1177/1535370220949237. Epub 2020 Aug 12.
Abstract
This review was conducted to present the main neuroblastoma (NB) clinical characteristics and the most common genetic alterations present in these pediatric tumors, highlighting their impact in tumor cell aggressiveness behavior, including metastatic development and treatment resistance, and patients' prognosis. The distinct three NB cell lineage phenotypes, S-type, N-type, and I-type, which are characterized by unique cell surface markers and gene expression patterns, are also reviewed. Finally, an overview of the most used NB cell lines currently available for studies and their unique cellular and molecular characteristics, which should be taken into account for the selection of the most appropriate model for NB pre-clinical studies, is presented. These valuable models can be complemented by the generation of NB reprogrammed tumor cells or organoids, derived directly from patients' tumor specimens, in the direction toward personalized medicine.
摘要
这篇综述旨在介绍神经母细胞瘤（NB）的主要临床特征和这些儿科肿瘤中常见的遗传改变，强调它们对肿瘤细胞侵袭性行为的影响，包括转移的发展和治疗耐药性以及患者的预后。还回顾了具有独特细胞表面标志物和基因表达模式的三种不同的 NB 细胞谱系表型，S 型、N 型和 I 型。最后，概述了目前用于研究的最常用的 NB 细胞系及其独特的细胞和分子特征，在选择最适合 NB 临床前研究的模型时应考虑这些特征。这些有价值的模型可以通过直接从患者肿瘤标本中衍生的 NB 重编程肿瘤细胞或类器官来补充，朝着个性化医疗的方向发展。
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