Cancer Research Laboratory, Methodist Research Institute, Indianapolis, IN 46202, USA.
Int J Mol Med. 2010 Nov;26(5):643-50. doi: 10.3892/ijmm_00000509.
Colorectal cancer is one of the leading causes of cancer deaths in both men and women in the world. However, colon cancer can be prevented to some extent by consumption of edible natural products with chemopreventive properties. Therefore, we investigated, whether edible mushroom Pleurotus ostreatus (PO) has chemopreventive effect on inflammation-associated colon carcinogenesis induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and promoted by dextran sodium sulfate (DSS). PO treatment, at both doses (100 and 500 mg/kg), significantly reduced by 50 and 78% the number of aberrant crypt foci and the multiplicity of colon neoplasms by 43 and 89%, respectively. However, incidence of colon tumors and high grade dysplasia was reduced by 50 and 63% only in the dose 500 mg/kg of PO, respectively. Colon shortening and dysplastic index was significantly reduced by PO treatment in dose-dependent manner. The immunohistochemistry of colons revealed that treatment with PO suppressed expression of cyclin D1, Ki-67, COX-2 and F4/80. In summary, our data suggest that PO may prevent inflammation-associated colon carcinogenesis with exposure to PhIP through combined modulatory mechanisms of inflammation and tumor growth via suppression of COX-2, F4/80, Ki-67 and cyclin D1 expression in mice.
结直肠癌是全世界男性和女性癌症死亡的主要原因之一。然而，通过食用具有化学预防特性的天然食品，可以在一定程度上预防结肠癌。因此，我们研究了食用蘑菇糙皮侧耳（PO）是否对 2-氨基-1-甲基-6-苯基咪唑[4,5-b]吡啶（PhIP）诱导的炎症相关结肠癌变和葡聚糖硫酸钠（DSS）促进的结肠癌有化学预防作用。PO 处理，在两个剂量（100 和 500 mg/kg）下，分别显著降低了 50%和 78%的异常隐窝病灶数量和结肠肿瘤的多发性，分别降低了 43%和 89%。然而，仅在 PO 剂量为 500 mg/kg 时，结肠肿瘤和高级别发育不良的发生率分别降低了 50%和 63%。PO 处理还以剂量依赖的方式显著降低了结肠缩短和发育不良指数。结肠的免疫组织化学显示，PO 处理抑制了 cyclin D1、Ki-67、COX-2 和 F4/80 的表达。总之，我们的数据表明，PO 可能通过抑制 COX-2、F4/80、Ki-67 和 cyclin D1 的表达，通过抑制炎症和肿瘤生长的联合调节机制，预防 PhIP 暴露引起的炎症相关结肠癌变。
