National Center for Gene Research and Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, 200233, China.
Theor Appl Genet. 2011 Feb;122(2):327-40. doi: 10.1007/s00122-010-1449-8. Epub 2010 Sep 28.
Mapping chromosome regions responsible for quantitative phenotypic variation in recombinant populations provides an effective means to characterize the genetic basis of complex traits. We conducted a quantitative trait loci (QTL) analysis of 150 rice recombinant inbred lines (RILs) derived from a cross between two cultivars, Oryza sativa ssp. indica cv. 93-11 and Oryza sativa ssp. japonica cv. Nipponbare. The RILs were genotyped through next-generation sequencing, which accurately determined the recombination breakpoints and provided a new type of genetic markers, recombination bins, for QTL analysis. We detected 49 QTL with phenotypic effect ranging from 3.2 to 46.0% for 14 agronomics traits. Five QTL of relatively large effect (14.6-46.0%) were located on small genomic regions, where strong candidate genes were found. The analysis using sequencing-based genotyping thus offers a powerful solution to map QTL with high resolution. Moreover, the RILs developed in this study serve as an excellent system for mapping and studying genetic basis of agricultural and biological traits of rice.
对重组群体中负责数量表型变异的染色体区域进行定位，为研究复杂性状的遗传基础提供了一种有效的方法。我们对来自籼稻品种 93-11 和粳稻品种 Nipponbare 杂交的 150 个水稻重组自交系（RIL）进行了数量性状位点（QTL）分析。通过下一代测序对 RIL 进行了基因型分析，该技术准确确定了重组断点，并为 QTL 分析提供了一种新的遗传标记——重组 bin。我们检测到了 49 个 QTL，它们对 14 个农艺性状的表型效应范围为 3.2%至 46.0%。5 个效应较大（14.6%至 46.0%）的 QTL位于小的基因组区域，在这些区域中发现了强候选基因。基于测序的基因型分析为高分辨率定位 QTL 提供了一种强大的解决方案。此外，本研究中开发的 RIL 为水稻农业和生物学性状的遗传基础的定位和研究提供了一个极好的系统。
Zotero 插件
