Ochiai K, Uemura S, Shimizu A, Okumoto Y, Matoh T
Laboratory of Plant Nutrition, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Theor Appl Genet. 2008 Jun;117(1):125-33. doi: 10.1007/s00122-008-0758-7. Epub 2008 Apr 10.
Boron toxicity tolerance of rice plants was studied. Modern japonica subspecies such as Koshihikari, Nipponbare, and Sasanishiki were tolerant, whereas indica subspecies such as Kasalath and IR36 were intolerant to excessive application of boron (B), even though their shoot B contents under B toxicity were not significantly different. Recombinant inbred lines (RILs) of japonica Nekken-1 and indica IR36 were used for quantitative trait locus (QTL) analysis to identify the gene responsible for B toxicity tolerance. A major QTL that could explain 45% of the phenotypic variation was detected in chromosome 4. The QTL was confirmed using a population derived from a recombinant inbred line which is heterogenic at the QTL region. The QTL was also confirmed in other chromosome segment substitution lines (CSSLs).
对水稻植株的硼毒性耐受性进行了研究。越光、日本晴和笹锦等现代粳稻亚种具有耐受性，而卡萨拉斯和IR36等籼稻亚种对过量施用硼（B）不耐受，尽管它们在硼毒性下的地上部硼含量没有显著差异。利用粳稻根健1号和籼稻IR36的重组自交系（RILs）进行数量性状位点（QTL）分析，以鉴定负责硼毒性耐受性的基因。在第4号染色体上检测到一个可解释45%表型变异的主要QTL。使用来自在QTL区域具有异质性的重组自交系的群体对该QTL进行了验证。该QTL在其他染色体片段代换系（CSSLs）中也得到了验证。
