Department of Clinical Laboratory, Peking University People's Hospital, Xicheng District, Beijing, China.
Arch Virol. 2011 Jan;156(1):17-23. doi: 10.1007/s00705-010-0811-0. Epub 2010 Sep 28.
Human cytomegalovirus (HCMV) genomic polymorphisms have been used to investigate correlations between virus variants and clinical characteristics. We explored the distribution of HCMV glycoprotein N (gN) genotypes and their roles relative to clinical features in a population of Chinese hematopoietic stem cell transplant (HSCT) recipients. This prospective analysis involved HCMV clinical isolates obtained from 102 HSCT patients. Real-time quantitative PCR and PCR-based restriction fragment length polymorphism analysis were applied for the determination of viral loads and gN genotypes. The distribution of HCMV gN genotypes was as follows: gN1, 6/102 (5.9%); gN2, 10/102 (9.8%); gN3a, 17/102 (16.7%); gN3b, 5/102 (4.9%); gN4a, 12/102 (11.7%); gN4b, 9/102 (8.8%); gN4d, 2/102 (2.0%); and mixtures, 41/102 (40.2%). No particular HCMV gN genotype was significantly associated with specific clinical characteristics. The HCMV gN3a genotype was the most prevalent among Chinese HSCT recipients, but HCMV gN genotypes may have no correlation with clinical features in HSCT patients.
人巨细胞病毒（HCMV）基因组多态性已被用于研究病毒变异体与临床特征之间的相关性。我们在一组中国造血干细胞移植（HSCT）受者中探索了 HCMV 糖蛋白 N（gN）基因型的分布及其与临床特征的关系。这项前瞻性分析涉及从 102 名 HSCT 患者中获得的 HCMV 临床分离株。实时定量 PCR 和基于 PCR 的限制性片段长度多态性分析用于确定病毒载量和 gN 基因型。HCMV gN 基因型的分布如下：gN1，6/102（5.9%）；gN2，10/102（9.8%）；gN3a，17/102（16.7%）；gN3b，5/102（4.9%）；gN4a，12/102（11.7%）；gN4b，9/102（8.8%）；gN4d，2/102（2.0%）和混合物，41/102（40.2%）。没有特定的 HCMV gN 基因型与特定的临床特征有显著相关性。HCMV gN3a 基因型在中国 HSCT 受者中最为常见，但 HCMV gN 基因型与 HSCT 患者的临床特征可能没有相关性。
