Department of Clinical Laboratory, Children's Hospital of Fudan University, Shanghai, China.
J Med Virol. 2020 Dec;92(12):3674-3681. doi: 10.1002/jmv.26210. Epub 2020 Jul 2.
Human cytomegalovirus (HCMV) is the leading cause of congenital infection and an opportunistic pathogen capable of establishing lifelong latency. In the present study, we aimed to investigate the distribution of glycoprotein B, H, and N in infants of Shanghai and correlate the genotype with active and latent HCMV infection. A total of 129 urine samples were collected between August 2014 and December 2015 from infants under 3 years with HCMV infection. Nested PCR was used to amplify the regions of UL55 (gB), UL75 (gH), and UL73 (gN). Gene sequencing and phylogenetic analyses were used to classify the genotypes. Overall, regarding gB, gB1 (57.27%) was predominant, followed by gB3 (41.82%) and gB4 (0.91%). gH1 (54.33%) was the most prevalent genotype of gH, followed by gH2 (45.67%). Concerning gN, we detected gN1 (17.44%), gN2 (2.33%), gN3a (29.07%), gN3b (8.14%), gN4a (13.95%), gN4b (15.12%), and gN4c (13.95%), among which gN3a was the dominant genotype. All the expected genotypes were present except gB2 in children with active infection: gB1 (56.25%), gB3 (42.5%), gB4 (1.25%), gH1 (58.70%), gH2 (41.30%), gN1 (19.05%), gN2 (3.17%), gN3a (25.40%), gN3b (6.35%), gN4a (15.87%), gN4b (17.46%), and gN4c (12.70%). However, among latent cases, we detected gB1 (60%), gB3 (40%), gH1 (42.86%), gH2 (57.14%), gN1 (13.04%), gN3a (39.13%), gN3b (13.04%), gN4a (8.70%), gN4b (8.70%), and gN4c (17.39%), respectively. gB2, gB4, and gN2 were absent in this group. The results revealed that gB1, gH1, and gN3a were predominant in the infants of Shanghai. gH showed different trends among children with active and latent infection.
人巨细胞病毒（HCMV）是导致先天性感染的主要原因，也是一种能够建立终身潜伏的机会性病原体。本研究旨在探讨上海地区婴幼儿中糖蛋白 B、H 和 N 的分布情况，并将基因型与 HCMV 感染的活动期和潜伏期相关联。我们收集了 2014 年 8 月至 2015 年 12 月间 3 岁以下患有 HCMV 感染的婴幼儿共 129 例尿液样本。采用巢式 PCR 扩增 UL55（gB）、UL75（gH）和 UL73（gN）区域。基因测序和系统进化分析用于对基因型进行分类。总体而言，gB 中以 gB1（57.27%）为主，gB3（41.82%）次之，gB4（0.91%）最少。gH1（54.33%）是 gH 最常见的基因型，gH2（45.67%）次之。gN 中，我们检测到 gN1（17.44%）、gN2（2.33%）、gN3a（29.07%）、gN3b（8.14%）、gN4a（13.95%）、gN4b（15.12%）和 gN4c（13.95%），其中 gN3a 是主要的基因型。在活动期感染的儿童中，除 gB2 外，均存在所有预期的基因型：gB1（56.25%）、gB3（42.5%）、gB4（1.25%）、gH1（58.70%）、gH2（41.30%）、gN1（19.05%）、gN2（3.17%）、gN3a（25.40%）、gN3b（6.35%）、gN4a（15.87%）、gN4b（17.46%）和 gN4c（12.70%）。然而，在潜伏期病例中，我们检测到 gB1（60%）、gB3（40%）、gH1（42.86%）、gH2（57.14%）、gN1（13.04%）、gN3a（39.13%）、gN3b（13.04%）、gN4a（8.70%）、gN4b（8.70%）和 gN4c（17.39%）。在这组中未检测到 gB2、gB4 和 gN2。结果表明，gB1、gH1 和 gN3a 在上海地区婴幼儿中占主导地位。gH 在活动期和潜伏期感染儿童中的表现趋势不同。
