Programa de Pós-Graduação em Ciências Morfológicas, Instituto de Ciências Biomédicas, Bloco F2-012, CCS, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21949-902, Rio de Janeiro, RJ, Brazil.
Cell Tissue Res. 2010 Nov;342(2):179-89. doi: 10.1007/s00441-010-1045-x. Epub 2010 Sep 28.
In a previous study, we analyzed and described the features of the degeneration of the protocerebral tract (PCT) of the crustacean Ucides cordatus, after the extirpation of the eyestalk. In that study, among axons with axoplasmic degeneration, cells with granules resembling blood cells (hemocytes) were seen. Therefore, in the present study, we characterized the circulating hemocytes and compared them with the cells recruited to a lesion, which was produced as in the former study. Using histochemistry, immunohistochemistry, and electron microscopy (transmission and scanning), we confirmed that circulating and recruited cells display a similar morphology. Therefore, in the crab, hemocytes were attracted to the lesion site in the acute stage of degeneration, appearing near local glial cells that showed signs of being responsive. Some of the attracted hemocytes displayed a morphology that was considered to be possibly activated blood cells. Also, the cells that migrated to the injured PCT displayed features, such as the presence of hydrolytic enzymes and an ability to phagocytize neural debris, similar to those of vertebrates. In summary, our results indicate that hemocytes were not only phagocytizing neural debris together with glial cells but also that they may be concerned with creating a favorable environment for regenerating events.
在之前的研究中，我们分析并描述了甲壳类动物 Ucides cordatus 眼柄切除后原脑径路（PCT）退化的特征。在该研究中，在轴突出现轴浆退行性变的同时，观察到了类似于血细胞（血球）的颗粒细胞。因此，在本研究中，我们对循环血球进行了特征描述，并将其与前研究中产生的病变募集细胞进行了比较。通过组织化学、免疫组织化学和电子显微镜（透射和扫描），我们证实循环和募集的细胞具有相似的形态。因此，在蟹类中，血球在退行性变的急性期被吸引到病变部位，出现在表现出反应性迹象的局部神经胶质细胞附近。一些被吸引的血球表现出可能被激活的血细胞的形态。此外，迁移到受损 PCT 的细胞表现出与脊椎动物相似的特征，如存在水解酶和吞噬神经碎片的能力。总之，我们的结果表明，血球不仅与神经胶质细胞一起吞噬神经碎片，而且可能与为再生事件创造有利环境有关。
