盘基网柄菌中的趋化作用：伴刀豆球蛋白A对趋化因子介导的环鸟苷酸积累和光散射减少的影响。

Chemotaxis in Dictyostelium discoideum: effect of concanavalin A on chemoattractant mediated cyclic gmp accumulation anlight scattering decrease.

作者信息

Mato J M, van Haastert P J, Krens F A, Konijn T M

出版信息

Cell Biol Int Rep. 1978 Mar;2(2):163-70. doi: 10.1016/0309-1651(78)90037-1.

PMID:208784

Abstract

In cells of the cellular slime mold Dictyostelium discoideum concanavalin A (Con A), at a concentration of 100 microgram per ml, inhibits folic acid and cyclic AMP induced decrease in light scattering. Con A has no effect on folic acid mediated cyclic GMP accumulation and increases cyclic AMP mediated cyclic GMP accumulation two-fold. At a lower Con A concentration, 10 microgram per ml, changes in light scattering induced by folic acid are normal and cyclic AMP induces a monophasic instead of a biphasic response. The stimulatory effect of Con A on cyclic AMP mediated cyclic GMP accumulation is still observable at 10 microgram Con A per ml. When cells are repeatedly stimulated with cyclic AMP, a decrease in light scattering without being accompanied by changes in cyclic GMP concentration is observed. Based on these results a model for chemotaxis is proposed.

摘要

在细胞黏菌盘基网柄菌的细胞中，浓度为每毫升100微克的伴刀豆球蛋白A（Con A）可抑制叶酸和环磷酸腺苷（cAMP）诱导的光散射减少。Con A对叶酸介导的环磷酸鸟苷（cGMP）积累没有影响，却能使cAMP介导的cGMP积累增加两倍。在较低的Con A浓度下，即每毫升10微克时，叶酸诱导的光散射变化正常，而cAMP诱导的是单相反应而非双相反应。每毫升10微克Con A时，Con A对cAMP介导的cGMP积累的刺激作用仍然可以观察到。当细胞用cAMP反复刺激时，会观察到光散射减少，且不伴有cGMP浓度变化。基于这些结果，提出了一种趋化性模型。