Cassoly R, Daveloose D, Wolf C, Leterrier F
C R Acad Hebd Seances Acad Sci D. 1978 Mar 28;286(12):1009-12.
Spectrin isolated from human erythrocytes has been spin-labeled with five maleimide nitroxides. The mobility of the labels is strongly dependent on their size, and on the temperature. A thermal transition of spectrin is shown to occur above 30 degrees C. Calcium and magnesium provoke a strong immobilization of the labels. This effect is yet more pronounced when spectrin is allowed to reassociate with the cytoplasmic surface of the membrane.
从人红细胞中分离出的血影蛋白已用五种马来酰亚胺氮氧化物进行了自旋标记。标记物的流动性在很大程度上取决于它们的大小和温度。血影蛋白在30摄氏度以上会发生热转变。钙和镁会使标记物强烈固定。当血影蛋白与膜的细胞质表面重新结合时，这种效应更为明显。
