Formation of DNA adducts in liver of rats treated with 2-aminofluorene. Differences between total and matrix attached DNA fractions.

The level of adducts in total DNA and the nuclear matrix-attached DNA was compared at 12, 24, 48 and 72 h after a single injection of a hepatocarcinogen, 2-aminofluorene. In rat liver, the amount of adducts in total DNA increased gradually up to 72 h, reaching the value of about 54 per 10(7) nucleotides, whereas the maximal level of adducts in matrix DNA was observed 48 h after injection. Matrix and total DNA differed, also in the level of adducts in particular spots in four dimensional chromatography on PEI cellulose plates.