用2-氨基芴处理的大鼠肝脏中DNA加合物的形成。总DNA组分与基质附着DNA组分之间的差异。
Formation of DNA adducts in liver of rats treated with 2-aminofluorene. Differences between total and matrix attached DNA fractions.
作者信息
Widłak P, Rzeszowska-Wolny J
机构信息
Institute of Oncology, Department of Tumour Biology, Gliwice, Poland.
出版信息
Acta Biochim Pol. 1990;37(1):65-7.
The level of adducts in total DNA and the nuclear matrix-attached DNA was compared at 12, 24, 48 and 72 h after a single injection of a hepatocarcinogen, 2-aminofluorene. In rat liver, the amount of adducts in total DNA increased gradually up to 72 h, reaching the value of about 54 per 10(7) nucleotides, whereas the maximal level of adducts in matrix DNA was observed 48 h after injection. Matrix and total DNA differed, also in the level of adducts in particular spots in four dimensional chromatography on PEI cellulose plates.
在单次注射肝癌致癌物2-氨基芴后的12、24、48和72小时，对总DNA和核基质附着DNA中的加合物水平进行了比较。在大鼠肝脏中，总DNA中的加合物量在72小时前逐渐增加，达到每10⁷个核苷酸约54个加合物的水平，而基质DNA中加合物的最高水平在注射后48小时观察到。在PEI纤维素板上进行的二维色谱分析中，基质DNA和总DNA在特定位点的加合物水平也存在差异。
Zotero 插件