Camejo Daymi, Martí María C, Román Paloma, Ortiz Ana, Jiménez Ana
Department of Stress Biology and Plant Pathology, CEBAS-CSIC, P.O. Box 164, E-30100 Murcia, Spain.
J Agric Food Chem. 2010 Oct 27;58(20):11140-7. doi: 10.1021/jf102807t. Epub 2010 Sep 29.
Peach fruits were selected to study the protein pattern and antioxidant system as well as oxidative parameters such as superoxide radical and hydrogen peroxide accumulation, at two maturity stages, which were chosen for being suitable for the processing industry and fresh consumption. The proteins phosphoenolpyruvate carboxylase, sucrose synthase, and 1-aminocyclopropane-1-carboxylate oxidase, as well as the antioxidants glutathione synthetase and ascorbate peroxidase, appeared as new in the mature peach fruits. Activities of superoxide dismutase (SOD) and components of the ascorbate-glutathione cycle were also measured to explore their role in the two maturity stages studied. Changes in the SOD isoenzyme pattern and an increase in the activities of ascorbate peroxidase, monodehydroascorbate reductase, and glutathione reductase were observed in mature fruits, revealing an efficient system to cope with the oxidative process accompanying ripening.
选择桃子果实来研究其蛋白质模式、抗氧化系统以及氧化参数，如超氧阴离子自由基和过氧化氢的积累情况，研究选取了两个适合加工行业和鲜食的成熟阶段。磷酸烯醇式丙酮酸羧化酶、蔗糖合酶和1-氨基环丙烷-1-羧酸氧化酶等蛋白质，以及抗氧化剂谷胱甘肽合成酶和抗坏血酸过氧化物酶，在成熟桃子果实中呈现为新出现的物质。还测定了超氧化物歧化酶（SOD）的活性以及抗坏血酸-谷胱甘肽循环的组分，以探究它们在两个研究成熟阶段中的作用。在成熟果实中观察到SOD同工酶模式的变化以及抗坏血酸过氧化物酶、单脱氢抗坏血酸还原酶和谷胱甘肽还原酶活性的增加，这揭示了一个有效的系统来应对伴随成熟过程的氧化过程。
