建立一种基于猪流行性腹泻病毒 M 蛋白的 ELISA 方法用于病毒检测。

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.

Biotechnol Lett. 2011 Feb;33(2):215-20. doi: 10.1007/s10529-010-0420-8. Epub 2010 Sep 30.

A membrane (M), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The M gene of PEDV was expressed in Escherichia coli. The purified recombinant M protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the anti-PEDV-M antibody reacted with PEDV-infected cells. The antibody was utilized to develop an indirect ELISA to detect PEDV. Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive.

一种基于膜（M）和蛋白质的酶联免疫吸附试验（ELISA）被开发出来，用于检测猪流行性腹泻病毒（PEDV）。PEDV 的 M 基因在大肠杆菌中表达。纯化的重组 M 蛋白被用于免疫兔子以产生多克隆抗体。免疫荧光分析表明，抗 PEDV-M 抗体与感染 PEDV 的细胞发生反应。该抗体被用于开发间接 ELISA 来检测 PEDV。其他病毒，如猪传染性胃肠炎冠状病毒、禽传染性支气管炎冠状病毒、猪繁殖与呼吸综合征病毒、经典猪瘟病毒和猪伪狂犬病病毒，均无反应。