Antas Marta, Olech Monika, Szczotka-Bochniarz Anna
Department of Swine Diseases, National Veterinary Research Institute, 24-100 Puławy, Poland.
Department of Biochemistry, National Veterinary Research Institute, 24-100 Puławy, Poland.
J Vet Res. 2021 Jul 22;65(3):265-269. doi: 10.2478/jvetres-2021-0041. eCollection 2021 Sep.
Porcine epidemic diarrhoea virus (PEDV) of the family causes significant economic losses in the pig industry worldwide. Wild boars contribute to the transmission of different viral, bacterial and parasitic infections to livestock animals and humans. However, their role in the maintenance and transmission of PEDV has not been established.
In this study, blood and faecal samples from 157 wild boars were collected from 14 provinces of Poland during the 2017-2018 hunting season. RNA was extracted from the faecal homogenate supernatant and subjected to quantitative RT-PCR (RT-qPCR), while clotted blood samples were used for detection of antibodies against PEDV by ELISA.
Five blood samples (3.2%) were seropositive in ELISA, while none of the faecal samples were found positive using RT-qPCR assays.
The results of this analysis indicate the need for additional studies incorporating a larger number of samples and preferably comparing different serological methods, to confirm whether wild boars in Poland act as PEDV reservoirs.
该家族的猪流行性腹泻病毒（PEDV）在全球养猪业中造成了重大经济损失。野猪会将不同的病毒、细菌和寄生虫感染传播给家畜和人类。然而，它们在PEDV的维持和传播中的作用尚未明确。
在本研究中，于2017 - 2018年狩猎季节从波兰14个省份采集了157头野猪的血液和粪便样本。从粪便匀浆上清液中提取RNA，并进行定量逆转录聚合酶链反应（RT-qPCR），而凝血样本则用于通过酶联免疫吸附测定（ELISA）检测抗PEDV抗体。
ELISA检测中5份血液样本（3.2%）呈血清阳性，而使用RT-qPCR检测未发现粪便样本呈阳性。
该分析结果表明需要进行更多研究，纳入更多样本，最好比较不同的血清学方法，以确认波兰的野猪是否为PEDV储存宿主。
