病毒诱导细胞转化抑制剂5'-脱氧-5'-S-异丁硫基腺苷的一些代谢产物的鉴定

Identification of some metabolic products of 5' -deoxy-5' -S-isobutylthioadenosine, an inhibitor of virus-induced cell transformation.

作者信息

Lawrence F, Richou M, Vedel M, Farrugia G, Blanchard P, Robert-Gero M

出版信息

Eur J Biochem. 1978 Jun 15;87(2):257-63. doi: 10.1111/j.1432-1033.1978.tb12374.x.

DOI:10.1111/j.1432-1033.1978.tb12374.x

PMID:208841

Abstract

5' -Deoxy-5' -S-isobutylthioadenosine (iBuS)5' Ado has been shown to be rapidly degraded to 5-deoxy-5-S-isobutylthioribose and adenine in procaryotes. In chick embryo fibroblasts there are two metabolic pathways for (iBuS)5' Ado degradation: (a) oxidative deamination into 5' -deoxy-5'-S-isobutylthioinosine (the main product) and (b) hydrolysis into 5-deoxy-5-S-isobutylthioribose plus adenine. The latter reaction is not due to bacterial contamination, since the same results were obtained under sterile conditions and in chick embryo fibroblasts in culture. The inhibition of the virus-induced cell transformation reported by us previously was due to (iBuS)5' Ado rather than to the main metabolic product of this molecule in chick embryo fibroblasts.

摘要

5'-脱氧-5'-S-异丁硫基腺苷（iBuS）5'腺苷已被证明在原核生物中会迅速降解为5-脱氧-5-S-异丁硫基核糖和腺嘌呤。在鸡胚成纤维细胞中，（iBuS）5'腺苷的降解有两条代谢途径：（a）氧化脱氨生成5'-脱氧-5'-S-异丁硫基肌苷（主要产物），以及（b）水解生成5-脱氧-5-S-异丁硫基核糖加腺嘌呤。后一种反应并非由细菌污染引起，因为在无菌条件下以及在培养的鸡胚成纤维细胞中都得到了相同的结果。我们之前报道的病毒诱导的细胞转化抑制作用是由于（iBuS）5'腺苷，而非该分子在鸡胚成纤维细胞中的主要代谢产物。