Lawrence F, Richou M, Robert-Gero M
Eur J Biochem. 1980 Jun;107(2):467-73. doi: 10.1111/j.1432-1033.1980.tb06052.x.
The rate of deamination of 5'-deoxy-5'-S-isobutylthioadenosine [(iBuS5'Ado] in chick embryo fibroblasts was substantially reduced after their infection and morphological transformation by Rous sarcoma virus. Concomitant with the reduction in rate of (iBuS)5'Ado deamination there was a decrease in adenosine deaminase and 5'-adenylic acid deaminase activities. The drop of these activities was related to infection and not to the expression of the src gene. (iBuS)5'Ado was deaminated by at least three enzymes or isoenzymes whose apparent molecular weights have been estimated to be 295000, 121000 and 37000 respectively. Two of these enzymes have been characterized as 5'-adenylic acid deaminase and the heavy form of adenosine deaminase, respectively.
鸡胚成纤维细胞在被劳氏肉瘤病毒感染并发生形态转化后，5'-脱氧-5'-S-异丁硫基腺苷[（iBuS）5'Ado]的脱氨速率大幅降低。伴随着（iBuS）5'Ado脱氨速率的降低，腺苷脱氨酶和5'-腺苷酸脱氨酶的活性也有所下降。这些活性的下降与感染有关，而非与src基因的表达有关。（iBuS）5'Ado可被至少三种酶或同工酶脱氨，其表观分子量分别估计为295000、121000和37000。其中两种酶分别被鉴定为5'-腺苷酸脱氨酶和腺苷脱氨酶的重形式。
