使用博尔顿-亨特试剂对量子点进行放射性碘化及生物分布研究

Radioiodination and biodistribution of quantum dots using Bolton-Hunter reagent.

作者信息

Park Jae Jun, Lee Tae Sup, Kang Joo Hyun, Song Rita, Cheon Gi Jeong

机构信息

Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), 215-4 Gongneung-Dong, Nowon-Gu, Seoul 139-706, Republic of Korea.

出版信息

Appl Radiat Isot. 2011 Jan;69(1):56-62. doi: 10.1016/j.apradiso.2010.09.004. Epub 2010 Sep 18.

DOI:10.1016/j.apradiso.2010.09.004

PMID:20888778

Abstract

In this study, the radioiodination and biodistribution of quantum dots (QDs) using Bolton-Hunter reagent were investigated. Radioiodination yield was 33.4 ± 2.0%. Fluorescent intensity of radioiodinated QDs decreased to 75.4% of the maximum prior to radioiodination. In biodistribution and ex vivo fluorescence imaging, radioiodinated QDs were highly accumulated in reticuloendothelial system (liver and spleen) and had low level bone uptakes and slow clearance from body. These results suggest that the radioiodination method of nanoparticles using Bolton-Hunter reagent could be easily used in the biodistribution and quantification of nanoparticles in vivo.

摘要

在本研究中，对使用博尔顿-亨特试剂进行量子点（QDs）的放射性碘化及生物分布进行了研究。放射性碘化产率为33.4±2.0%。放射性碘化量子点的荧光强度降至碘化前最大值的75.4%。在生物分布和离体荧光成像中，放射性碘化量子点在网状内皮系统（肝脏和脾脏）中高度蓄积，骨摄取水平低，且从体内清除缓慢。这些结果表明，使用博尔顿-亨特试剂对纳米颗粒进行放射性碘化的方法可轻松用于纳米颗粒在体内的生物分布和定量研究。