苏氨酸 171 参与苏云金芽孢杆菌 Cry1Ab 德尔塔内毒素 α-螺旋 5 区在毒素向舞毒蛾中肠膜转位中的作用。
Participation of valine 171 in alpha-Helix 5 of Bacillus thuringiensis Cry1Ab delta-endotoxin in translocation of toxin into Lymantria dispar midgut membranes.
机构信息
Biochemistry Department, The Ohio State University, 484 W. 12th Avenue, Columbus, Ohio 43210, USA.
出版信息
Appl Environ Microbiol. 2010 Dec;76(23):7878-80. doi: 10.1128/AEM.01428-10. Epub 2010 Oct 1.
Abstract
The Cry1Ab δ-endotoxin V171C mutant protein exhibits a 25-fold increase in toxicity against Lymantria dispar, which correlates with a faster rate of partitioning into the midgut membrane and slightly decreased protein stability. This is an insect-specific mechanism; similar results were not observed in Manduca sexta, another Cry1Ab δ-endotoxin-susceptible insect.
摘要
Cry1Ab δ-内毒素 V171C 突变蛋白对舞毒蛾的毒性增加了 25 倍，这与更快的分配到中肠膜的速度和稍微降低的蛋白质稳定性有关。这是一种昆虫特异性机制；在另一种 Cry1Ab δ-内毒素敏感昆虫烟青虫中，没有观察到类似的结果。
相似文献
1
Participation of valine 171 in alpha-Helix 5 of Bacillus thuringiensis Cry1Ab delta-endotoxin in translocation of toxin into Lymantria dispar midgut membranes.
Appl Environ Microbiol. 2010 Dec;76(23):7878-80. doi: 10.1128/AEM.01428-10. Epub 2010 Oct 1.
2
Ser170 of Bacillus thuringiensis Cry1Ab delta-endotoxin becomes anchored in a hydrophobic moiety upon insertion of this protein into Manduca sexta brush border membranes.
BMC Biochem. 2009 Oct 19;10:25. doi: 10.1186/1471-2091-10-25.
3
Role of two arginine residues in domain II, loop 2 of Cry1Ab and Cry1Ac Bacillus thuringiensis delta-endotoxin in toxicity and binding to Manduca sexta and Lymantria dispar aminopeptidase N.
Mol Microbiol. 2000 Oct;38(2):289-98. doi: 10.1046/j.1365-2958.2000.02109.x.
4
Role of alkaline phosphatase from Manduca sexta in the mechanism of action of Bacillus thuringiensis Cry1Ab toxin.
J Biol Chem. 2010 Apr 23;285(17):12497-503. doi: 10.1074/jbc.M109.085266. Epub 2010 Feb 22.
5
Dominant negative phenotype of Bacillus thuringiensis Cry1Ab, Cry11Aa and Cry4Ba mutants suggest hetero-oligomer formation among different Cry toxins.
PLoS One. 2011;6(5):e19952. doi: 10.1371/journal.pone.0019952. Epub 2011 May 16.
6
The pre-pore from Bacillus thuringiensis Cry1Ab toxin is necessary to induce insect death in Manduca sexta.
Peptides. 2008 Feb;29(2):318-23. doi: 10.1016/j.peptides.2007.09.026. Epub 2007 Dec 14.
7
Mutations in domain I interhelical loops affect the rate of pore formation by the Bacillus thuringiensis Cry1Aa toxin in insect midgut brush border membrane vesicles.
Appl Environ Microbiol. 2009 Jun;75(12):3842-50. doi: 10.1128/AEM.02924-08. Epub 2009 Apr 17.
8
Mutations at the arginine residues in alpha8 loop of Bacillus thuringiensis delta-endotoxin Cry1Ac affect toxicity and binding to Manduca sexta and Lymantria dispar aminopeptidase N.
FEBS Lett. 2001 May 25;497(2-3):108-12. doi: 10.1016/s0014-5793(01)02446-2.
9
Bacillus thuringiensis Cry1Ab mutants affecting oligomer formation are non-toxic to Manduca sexta larvae.
J Biol Chem. 2007 Jul 20;282(29):21222-9. doi: 10.1074/jbc.M701314200. Epub 2007 May 30.
10
Modified Bacillus thuringiensis toxins and a hybrid B. thuringiensis strain counter greenhouse-selected resistance in Trichoplusia ni.
Appl Environ Microbiol. 2009 Sep;75(17):5739-41. doi: 10.1128/AEM.00664-09. Epub 2009 Jul 10.
引用本文的文献
1
Genome mining of Bacillus thuringiensis strain SY49.1 reveals novel candidate pesticidal and bioactive compounds.
Pest Manag Sci. 2025 Jan;81(1):298-307. doi: 10.1002/ps.8433. Epub 2024 Sep 26.
2
Toxic Determination of Cry11 Mutated Proteins Obtained Using Rational Design and Its Computational Analysis.
Int J Mol Sci. 2023 May 22;24(10):9079. doi: 10.3390/ijms24109079.
3
Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action.
Toxins (Basel). 2020 Sep 16;12(9):600. doi: 10.3390/toxins12090600.
4
Generation of Cry11 Variants of by Heuristic Computational Modeling.
Evol Bioinform Online. 2020 Jul 27;16:1176934320924681. doi: 10.1177/1176934320924681. eCollection 2020.
5
A transgenic approach for controlling Lygus in cotton.
Nat Commun. 2016 Jul 18;7:12213. doi: 10.1038/ncomms12213.
6
Chronic Responses of Daphnia magna Under Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt-Maize Hybrid and its Conventional Near-Isoline.
J Toxicol Environ Health A. 2015;78(15):993-1007. doi: 10.1080/15287394.2015.1037877.
7
Molecular approaches to improve the insecticidal activity of Bacillus thuringiensis Cry toxins.
Toxins (Basel). 2014 Aug 13;6(8):2393-423. doi: 10.3390/toxins6082393.
8
Evolution of Bacillus thuringiensis Cry toxins insecticidal activity.
Microb Biotechnol. 2013 Jan;6(1):17-26. doi: 10.1111/j.1751-7915.2012.00342.x. Epub 2012 Mar 29.
本文引用的文献
1
Ser170 of Bacillus thuringiensis Cry1Ab delta-endotoxin becomes anchored in a hydrophobic moiety upon insertion of this protein into Manduca sexta brush border membranes.
BMC Biochem. 2009 Oct 19;10:25. doi: 10.1186/1471-2091-10-25.
2
Membrane insertion of the Bacillus thuringiensis Cry1Ab toxin: single mutation in domain II block partitioning of the toxin into the brush border membrane.
Biochemistry. 2008 May 27;47(21):5814-22. doi: 10.1021/bi7014234. Epub 2008 May 6.
3
Effect of insect larval midgut proteases on the activity of Bacillus thuringiensis Cry toxins.
Appl Environ Microbiol. 2007 Oct;73(19):6208-13. doi: 10.1128/AEM.01188-07. Epub 2007 Aug 10.
4
Effects of disulfide bridges in domain I of Bacillus thuringiensis Cry1Aa delta-endotoxin on ion-channel formation in biological membranes.
Biochemistry. 2006 Nov 14;45(45):13597-605. doi: 10.1021/bi061474z.
5
Redesigning Bacillus thuringiensis Cry1Aa toxin into a mosquito toxin.
Protein Eng Des Sel. 2006 Mar;19(3):107-11. doi: 10.1093/protein/gzj009. Epub 2006 Jan 25.
6
Role of alpha-helix seven of Bacillus thuringiensis Cry1Ab delta-endotoxin in membrane insertion, structural stability, and ion channel activity.
Biochemistry. 2001 Feb 27;40(8):2540-7. doi: 10.1021/bi0022240.
7
Production of chymotrypsin-resistant Bacillus thuringiensis Cry2Aa1 delta-endotoxin by protein engineering.
Appl Environ Microbiol. 1999 Oct;65(10):4601-5. doi: 10.1128/AEM.65.10.4601-4605.1999.
8
Bacillus thuringiensis and its pesticidal crystal proteins.
Microbiol Mol Biol Rev. 1998 Sep;62(3):775-806. doi: 10.1128/MMBR.62.3.775-806.1998.
9
Cysteine-scanning mutagenesis of transmembrane domain XII and the flanking periplasmic loop in the lactose permease of EScherichia coli.
Biochemistry. 1996 Oct 1;35(39):12909-14. doi: 10.1021/bi960876b.
10
Role of domain II, loop 2 residues of Bacillus thuringiensis CryIAb delta-endotoxin in reversible and irreversible binding to Manduca sexta and Heliothis virescens.
J Biol Chem. 1996 Feb 2;271(5):2390-6. doi: 10.1074/jbc.271.5.2390.
Zotero 插件