Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler D R, Dean D H
Mycogen Corp., San Diego, California 92121, USA.
Microbiol Mol Biol Rev. 1998 Sep;62(3):775-806. doi: 10.1128/MMBR.62.3.775-806.1998.
During the past decade the pesticidal bacterium Bacillus thuringiensis has been the subject of intensive research. These efforts have yielded considerable data about the complex relationships between the structure, mechanism of action, and genetics of the organism's pesticidal crystal proteins, and a coherent picture of these relationships is beginning to emerge. Other studies have focused on the ecological role of the B. thuringiensis crystal proteins, their performance in agricultural and other natural settings, and the evolution of resistance mechanisms in target pests. Armed with this knowledge base and with the tools of modern biotechnology, researchers are now reporting promising results in engineering more-useful toxins and formulations, in creating transgenic plants that express pesticidal activity, and in constructing integrated management strategies to insure that these products are utilized with maximum efficiency and benefit.
在过去十年中,杀虫细菌苏云金芽孢杆菌一直是深入研究的对象。这些研究工作已经产生了大量有关该生物体杀虫晶体蛋白的结构、作用机制和遗传学之间复杂关系的数据,并且这些关系的连贯图景正开始显现。其他研究则集中在苏云金芽孢杆菌晶体蛋白的生态作用、它们在农业和其他自然环境中的表现,以及目标害虫抗性机制的进化。有了这个知识库以及现代生物技术工具,研究人员现在在设计更有用的毒素和制剂、培育具有杀虫活性的转基因植物以及构建综合管理策略以确保这些产品得到最有效利用和带来最大益处方面报告了令人鼓舞的结果。