Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Opt Lett. 2010 Oct 1;35(19):3189-91. doi: 10.1364/OL.35.003189.
In this experimental investigation, we explore the feasibility of using wide-field illumination for time-resolved fluorescence molecular tomography. The performance of wide-field patterns with a time-resolved imaging platform is investigated in vitro and in a small animal model. A Monte Carlo-based forward model is employed to reconstruct fluorescence yield based on time-gated datasets. An improvement in resolution and quantification when using the time-gate data type compared to the commonly used cw data type is demonstrated in vitro. Furthermore, the feasibility of wide-field strategies for fluorescence preclinical applications is established by an accurate localization of a fluorescent inclusion implanted in the chest cavity of a murine model.
在这项实验研究中，我们探索了使用广角照明进行时间分辨荧光分子层析成像的可行性。我们在体外和小动物模型中研究了具有时间分辨成像平台的广角模式的性能。基于蒙特卡罗的正向模型用于基于时间门控数据集重建荧光产率。在体外实验中，与常用的连续波数据类型相比，使用时间门控数据类型时，分辨率和定量能力得到了提高。此外，通过对植入小鼠模型胸腔的荧光包裹体的精确定位，证实了广角策略在荧光临床前应用中的可行性。
