Micro-scale liquid chromatographic method for the determination of bamifylline and its major metabolite in human plasma.

A sensitive micro-scale method based on isocratic elution reversed-phase ion-pair chromatography for the determination of bamifylline and its major metabolite (AC 119) in human plasma is described. The method is based on a liquid-liquid extraction clean-up followed by analysis on an LC-Packings Fusica (Delta Pak, RP-18, 5 microns, 300 A) column (15 cm x 330 microns. I.D.) with 0.03 M heptanesulphonate (pH 3.5)-acetonitrile (7:3, v/v) as the mobile phase. Data with respect to recovery, reproducibility and limits of detection are reported and discussed.