Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Nov-Dec;2(6):578-600. doi: 10.1002/wnan.96.
Encoded anisotropic nano- and microparticles represent an exciting new class of detection and identification strategies for bioanalysis. These particles are synthesized in a number of different ways and can be encoded by shape, composition, topographical features, or optical properties. In this review, we explore synthetic methods for the formation of anisotropic encoded particles and evaluate these systems as multiplexed biosensing platforms. Suspension arrays using anisotropic particles have been used to detect a range of biological species including proteins, nucleic acids, spores, cells, and small molecules. Because in many cases a large number of codes should be obtainable, the potential exists for high levels of multiplexing (thousands or more). The bulk of work in this area to date has focused on initial proof of principle synthesis and identification; however, multiplexed bioassays have been demonstrated for a number of different anisotropic carrier particles and are beginning to be adopted in commercial assays.
编码各向异性纳米和微颗粒代表了一类用于生物分析的令人兴奋的新型检测和识别策略。这些颗粒可以通过多种不同的方法合成，并可以通过形状、组成、形貌特征或光学性质进行编码。在本综述中，我们探索了形成各向异性编码颗粒的合成方法，并将这些系统评估为多重生物传感平台。使用各向异性颗粒的悬浮阵列已被用于检测多种生物物种，包括蛋白质、核酸、孢子、细胞和小分子。由于在许多情况下可以获得大量的编码，因此存在高度的多重化（数千个或更多）的可能性。迄今为止，该领域的大部分工作都集中在最初的原理合成和识别的证明上；然而，已经针对许多不同的各向异性载体颗粒演示了多重生物测定，并且开始在商业测定中采用。
