Jolly S R, Lech J J, Menahan L A
Horm Metab Res. 1978 May;10(3):223-7. doi: 10.1055/s-0028-1093439.
The lipolytic response of isolated adipocytes from genetic obese (C57/BL/64 ob/ob) and lean (C57BL/6J +/?) mice to ACTH-(1-24), isoproterenol and glucagon has been studied. The mean cell idameter of adipocytes form ob/ob mice was approximately twice that of lean controls. The adipocytes from obese mice contained on the average approximately six times the amount of triacylglycerol present in the smaller lean mouse adipocyte. Lipolysis was calculated both on a per cell basis (10(5) cells) and per mu mole of triacylglycerol and when expressed on a cell number basis, the larger adipocytes from obese mice showed an ACTH-(1-24) stimulated glycerol release which was quantitatively similar to that of smaller adipocytes from lean mice. When expressed per mu mole of triacylglycerol, the smaller cells from lean animals appeared to be dramatically more responsive to either isoproterenol or ACTH-(1-24). On either basis, ACTH-(1-24) stimulated glycerol release from obese mouse cells was greater than the isoproterenol response. The obese mouse of adipocyte showed selective loss of response to isoproterenol compared to its lean control.
对遗传性肥胖（C57/BL/64 ob/ob）和瘦（C57BL/6J +/?）小鼠分离的脂肪细胞对促肾上腺皮质激素（1 - 24）、异丙肾上腺素和胰高血糖素的脂解反应进行了研究。ob/ob小鼠脂肪细胞的平均细胞直径约为瘦对照小鼠的两倍。肥胖小鼠的脂肪细胞平均含有约为较小的瘦小鼠脂肪细胞中三酰甘油含量六倍的三酰甘油。脂解作用以每细胞（10⁵个细胞）和每微摩尔三酰甘油为基础进行计算，当以细胞数量为基础表示时，肥胖小鼠的较大脂肪细胞显示出促肾上腺皮质激素（1 - 24）刺激的甘油释放，其在数量上与瘦小鼠的较小脂肪细胞相似。当以每微摩尔三酰甘油表示时，瘦动物的较小细胞对异丙肾上腺素或促肾上腺皮质激素（1 - 24）的反应似乎明显更强。无论以哪种方式表示，促肾上腺皮质激素（1 - 24）刺激肥胖小鼠细胞释放甘油的量都大于异丙肾上腺素的反应。与瘦对照相比，肥胖小鼠的脂肪细胞对异丙肾上腺素的反应出现选择性丧失。
