Massara C L, Costa H M, Carvalho O dos S
Centro de Pesquisas René Rachou/FIOCRUZ, Belo Horizonte, MG.
Rev Soc Bras Med Trop. 1990 Jan-Mar;23(1):43-7. doi: 10.1590/s0037-86821990000100008.
With the purpose of standardising techniques for the laboratory study of Ascaris lumbricoides, faeces were collected from children parasitised by A. lumbricoides, during a 24 hour period. The fecal samples were sieved and resuspended several times in water. The sediment containing the eggs was cultivated in H2SO4 0.1N in tissue culture flasks, at 28 degrees C. The culture of embryonated eggs was determined every ten days starting from the 20th day of culture achieving around 98.0% embryogeny on the 80th day of culture. The best day to recover larvae from mice was determined by infecting 9 groups of 5 mice per os with 200 embryonated eggs/mouse. Each mouse was sacrificed by cervical rupture starting on the 4 day up to the 12 day of infection. On the 8 day after the infection 0.45% of the larvae were recovered from the lungs. The inoculum determination was performed by using 5 groups of 10 infected mice with 200, 400, 800, 1,600 and 3,200 embryo eggs/mouse. The best age for recovery of infection was achieved by using 4 groups animals, with 5 mice/group, with age varying from 20 to 50 days and an inoculum of 3,200 eggs. The best recovery (1.9% was obtained from the group of 20 days of age.
为规范蛔虫实验室研究技术，在24小时内收集了感染蛔虫的儿童粪便。粪便样本经多次过筛并在水中重悬。将含虫卵的沉淀物置于0.1N硫酸中，在28℃的组织培养瓶中培养。从培养第20天开始，每10天测定一次感染性虫卵的培养情况，在培养第80天时胚胎发生率达到约98.0%。通过每组5只小鼠，经口感染每只小鼠200个感染性虫卵，共9组，来确定从小鼠体内回收幼虫的最佳时间。从感染第4天到第12天，每组小鼠通过颈椎脱臼处死。感染后第8天，从肺中回收了0.45%的幼虫。通过使用5组每组10只感染小鼠，分别感染每只小鼠200、400、800、1600和3200个胚胎卵，来进行接种量测定。通过使用4组动物，每组5只小鼠，年龄在20至50天之间，接种3200个卵，来确定最佳感染恢复年龄。从20日龄组获得了最佳回收率（1.9%）。
