Vizziano D, Berois N
Departamento de Biología Celular, Facultad de Humanidades y Ciencias, Montevideo, Uruguay.
Rev Bras Biol. 1990 May;50(2):523-36.
The reproductive cycle of Teleostean fishes may be studied from different points of view. One of them is to examine the histological changes that take place in the gonad. A histological description of the gonad is to be done first. In this work we have studied the oogenesis, atresia and post-ovulatory follicles of Macrodon ancylodon. Specimens were collected from April 1982 to May 1983 on the coast of Río de la Plata (Montevideo, Uruguay). Material used was preserved in neutralized 10% formalin inbedded in paraffin and paraffin-celloidin, sectioned at 5 - 10 microns and stained with haematoxilin-eosin. Pas-haematoxilin, and specific techniques for lipid detection were used. Six oogenetic stages were recognized: oogonias, and basophilic, lipid yolk vesicles, protein yolk vesicles, matures and ripe hydrated oocytes. Theca and granulosa are negative to lipid and cholesterol histochemic reaction techniques. Post-ovulatory follicles show structural degenerative changes. Two types of atresia are described: hypertrophic and nonhypertrophic, which apparently would not have an endocrine function. It is important to recognize post-ovulatory follicles to establish the spawning pattern and also to recognize atretic follicles due to their incidence in the fecundity of a species.
硬骨鱼类的生殖周期可以从不同角度进行研究。其中一个角度是检查性腺中发生的组织学变化。首先要对性腺进行组织学描述。在这项研究中，我们研究了大口犬牙南极鱼的卵子发生、闭锁卵泡和排卵后卵泡。样本采集于1982年4月至1983年5月，取自拉普拉塔河沿岸（乌拉圭蒙得维的亚）。所用材料保存在中和的10%福尔马林中，经石蜡和石蜡 - 火棉胶包埋，切成5 - 10微米厚的切片，并用苏木精 - 伊红、派洛宁 - 苏木精染色以及采用脂质检测的特定技术。识别出了六个卵子发生阶段：卵原细胞、嗜碱性脂质卵黄囊泡、蛋白质卵黄囊泡、成熟卵母细胞和成熟水化卵母细胞。卵泡膜和颗粒细胞对脂质和胆固醇组织化学反应技术呈阴性。排卵后卵泡呈现出结构退行性变化。描述了两种闭锁类型：肥大性闭锁和非肥大性闭锁，它们显然没有内分泌功能。识别排卵后卵泡对于确定产卵模式很重要，识别闭锁卵泡对于了解其在物种繁殖力中的发生率也很重要。
