Katayama Y, Becker D P, Tamura T, Tsubokawa T
Division of Neurosurgery, University of California, Los Angeles.
Acta Neurochir Suppl (Wien). 1990;51:183-5. doi: 10.1007/978-3-7091-9115-6_62.
When rapid cellular swelling occurs, water moves from the extracellular space (ECS) into the cells and the concentration of ECS markers which do not move into the cells increases. Cellular swelling during cerebral ischaemia has therefore been demonstrated in vivo as an increase in ECS markers which is measurable with intracerebral electrodes. We attempted to detect the cellular swelling by brain microdialysis employing a similar principle. Dialysis probes were placed in the hippocampus, and perfused for 20 min with 14C-sucrose as an ECS marker. The probes were subsequently perfused without 14C-sucrose and the dialysate concentration of 14C-sucrose was determined at 1-min intervals. The dialysate concentrations of 14C-sucrose suddenly became elevated 1-3 min after the onset of cerebral ischaemia, indicating the occurrence of cellular swelling. The present technique is useful because it enables the mechanism of cellular swelling to be analyzed by observing the effects of pharmacological agents administered through a dialysis probe. Preliminary data indicating the role of excitatory amino acids in producing cellular swelling during cerebral ischaemia are presented as an example.
当细胞迅速肿胀时，水从细胞外间隙（ECS）进入细胞，而未进入细胞的ECS标志物浓度增加。因此，脑缺血期间的细胞肿胀在体内表现为ECS标志物增加，这可以通过脑内电极进行测量。我们试图利用类似原理通过脑微透析检测细胞肿胀。将透析探针置于海马体中，用作为ECS标志物的14C-蔗糖灌注20分钟。随后在不使用14C-蔗糖的情况下对探针进行灌注，并每隔1分钟测定透析液中14C-蔗糖的浓度。脑缺血发作后1 - 3分钟，透析液中14C-蔗糖的浓度突然升高，表明发生了细胞肿胀。本技术很有用，因为它能够通过观察通过透析探针给药的药理剂的作用来分析细胞肿胀的机制。作为示例，给出了表明兴奋性氨基酸在脑缺血期间产生细胞肿胀中作用的初步数据。
