Hlinák Z, Krejcí I, Hondlík J, Yamamoto A
Research Institute for Pharmacy and Biochemistry, Prague, Czechoslovakia.
Methods Find Exp Clin Pharmacol. 1990 Jul-Aug;12(6):385-93.
The short-term effects and long-term consequences of sodium nitrite-induced hypoxia on spontaneous behavior of adult male rats were studied. A short-term suppression of locomotor, exploratory and grooming activities in 3-month-old animals after single NaNO2 does (55 mg per kg, s.c.) was followed by the recovery of normal values within 24 h. A marked decrease in locomotor and exploratory activity as well as of investigatory time combined with an increase of time spent in relaxation patterns was found in 6-month-old males at day 7 after double NaNO2 administration. A more rapid habituation during repeated exposure of 3-month-old males into the same experimental box was found beginning on day 10 after three-fold NaNO2 administration. We suggest that the delayed behavioral deficits could be due to the secondary structural changes developing in the central nervous system over several days after the NaNO2 insult. Alaptide, a dipeptide derived from Pro-Leu-Gly-NH2, administered for five days in a preventive-curative way ameliorated the deficits in spontaneous behavior consequent to the NaNO2 effect. At the moment it is not possible to fully understand the mechanism of action which is responsible for the beneficial effect of alaptide.
研究了亚硝酸钠诱导的缺氧对成年雄性大鼠自发行为的短期影响和长期后果。单次皮下注射亚硝酸钠（55毫克/千克）后，3个月大的动物的运动、探索和梳理活动出现短期抑制，24小时内恢复到正常值。在双次注射亚硝酸钠7天后，6个月大的雄性大鼠的运动和探索活动以及探究时间显著减少，同时放松模式下花费的时间增加。在三次注射亚硝酸钠10天后，发现3个月大的雄性大鼠在反复进入同一实验箱时习惯化速度加快。我们认为，延迟出现的行为缺陷可能是由于亚硝酸钠损伤后数天中枢神经系统发生的继发性结构变化所致。Alaptide是一种由Pro-Leu-Gly-NH2衍生的二肽，以预防-治疗方式给药5天，改善了亚硝酸钠作用导致的自发行为缺陷。目前尚无法完全理解负责Alaptide有益作用的作用机制。
