Akagawa K
Department of Neurochemistry, National Institute for Physiological Sciences, Aichi, Japan.
Brain Res Dev Brain Res. 1990 Dec 1;57(1):143-5. doi: 10.1016/0165-3806(90)90194-4.
Since histological studies on retinal reaggregates have given evidence of lamination similar to that in the intact retina, the presence of retinal function, i.e. light response, in vitro has been expected as well. Therefore, neurons in the outermost layers of reaggregates, mostly consisting of amacrine cells, were studied with cell-attached recording. About one fifth of the neurons in that layer showed spontaneous discharges. Interestingly, the frequency of spontaneous discharges of some neurons increased with light stimulation but decreased to the basal level under dark conditions. They were reproducible and lasted more than 10 min in the best case. These results suggest that functional connections between photoreceptors which are exclusively present in the central region and amacrine cells in the outermost layer might be reconstituted in reaggregate cultures.
由于对视网膜重聚体的组织学研究已经提供了类似于完整视网膜分层的证据，因此人们也期望体外存在视网膜功能，即光反应。因此，使用细胞贴附记录法对重聚体外层的神经元进行了研究，这些外层神经元主要由无长突细胞组成。该层中约五分之一的神经元表现出自发放电。有趣的是，一些神经元的自发放电频率在光刺激下增加，但在黑暗条件下降至基础水平。它们具有可重复性，在最佳情况下可持续超过10分钟。这些结果表明，仅存在于中央区域的光感受器与最外层无长突细胞之间的功能连接可能在重聚体培养物中得以重建。
