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通过扫描电子显微镜研究供体晶状体囊膜材料的形态学。

Morphology of donor lens-capsule material studied by SEM.

作者信息

Jongebloed W L, Los L I, Dijk F, Worst J G

机构信息

Department of Histology and Cellbiology, University of Groningen, The Netherlands.

出版信息

Doc Ophthalmol. 1990 Oct;75(3-4):343-50. doi: 10.1007/BF00164849.

DOI:10.1007/BF00164849
PMID:2090410
Abstract

The lens capsule of a 70-year-old male donor with a cataractous lens was carefully prepared for SEM by first washing the capsule with buffer solution to remove lens-fibres and subsequently attaching it to silicon rubber. During the fixation and drying stages of the preparation procedure the capsule stayed attached to the rubber substratum. In the equatorial zone germinating cells were found with knob-shaped microvilli, closely connected to lens-fibres. Large units of pathological capsule epithelial cells were found, only slightly inter connected by a few pseudopodia. In addition, single pathological epithelial cells with pseudopodia, arranged on top of the cell in a rosette-like configuration, were found at certain locations. Both types are probably related to the original lens-cataract.

摘要

一名患有白内障晶状体的70岁男性供体的晶状体囊,首先用缓冲溶液冲洗囊以去除晶状体纤维,随后将其附着在硅橡胶上,为扫描电子显微镜(SEM)做了仔细准备。在制备过程的固定和干燥阶段,囊一直附着在橡胶基质上。在赤道区域发现了带有球状微绒毛的发芽细胞,它们与晶状体纤维紧密相连。还发现了大量病理状态的囊上皮细胞单元,仅通过少数伪足轻微相连。此外,在某些位置发现了单个带有伪足的病理上皮细胞,它们以玫瑰花结样结构排列在细胞顶部。这两种类型可能都与原始的晶状体白内障有关。

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引用本文的文献

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Human capsule epithelial cell degeneration, A LM, SEM and TEM investigation.人晶状体上皮细胞退变:光镜、扫描电镜及透射电镜研究
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Morphological aspects of human lens capsules. A comparative LM, SEM and TEM examination.人晶状体囊膜的形态学研究。光镜、扫描电镜和透射电镜的对比观察
Doc Ophthalmol. 1991;78(3-4):317-24. doi: 10.1007/BF00165695.

本文引用的文献

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Scanning electron microscopic study of eye tissues.
J Am Intraocul Implant Soc. 1984 Spring;10(2):169-75. doi: 10.1016/s0146-2776(84)80102-0.
2
Stainless steel as suturing material in human- and rabbit corneas: a SEM-study.
Doc Ophthalmol. 1988 Oct-Nov;70(2-3):145-54. doi: 10.1007/BF00154447.
3
The role of scanning electron microscopy in ophthalmic science.扫描电子显微镜在眼科学中的作用。
Scanning Microsc. 1988 Sep;2(3):1695-723.
4
Lens-capsule material of human and animal origin, studied by SEM.
Doc Ophthalmol. 1989 Aug;72(3-4):357-65. doi: 10.1007/BF00153504.